MEM, suspension, no calcium, no glutamine
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MEM, suspension, no calcium, no glutamine
MEM, suspension, no calcium, no glutamine
MEM, suspension, no calcium, no glutamine
Gibco™

MEM, suspension, no calcium, no glutamine

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be usedRead more
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Catalog NumberQuantity
11380037500 mL
Catalog number 11380037
Price (CNY)
462.80
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Ends: 31-Dec-2025
590.00
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Quantity:
500 mL
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Price (CNY)
462.80
飞享价
Ends: 31-Dec-2025
590.00
Save 127.20 (22%)
Each
Add to cart

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco™ MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.


This MEM is modified as follows:
WithWithout
• Earle's salts• L-glutamine
• Phenol Red• HEPES
 • Calcium


The complete formulation is available.

MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. This MEM formulation is made without calcium for use with suspension cultures. This MEM formulation contains Earle’s salts for use in a CO2 incubator. This product is made with Earle’s salts.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law.

MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law. For in vitro diagnostic use.
Specifications
Cell LineHeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Cell TypePrimary Rat Astrocytes
Concentration1 X
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco
Product TypeMEM (Minimum Essential Medium)
Quantity500 mL
Shelf Life12 Months From Date of Manufacture
Shipping ConditionRoom Temperature
ClassificationAnimal Origin-free
FormLiquid
Serum LevelStandard Serum Supplementation
SterilitySterile-filtered
With AdditivesLow Glucose, Phenol Red, Earle's Balanced Salt Solution (EBSS)
Without AdditivesNo Glutamine, No HEPES, No Sodium Pyruvate, No Calcium
Unit SizeEach
Contents & Storage
Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Frequently asked questions (FAQs)

Where can I find the osmolality for MEM Medium?

The osmolality is listed in the COA for the particular lot number of the medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I see a decrease in growth of my culture. What should I do?

Try changing the medium or serum. Compare media formulations for differences in glucose, amino acids, and other components. Compare an old lot of serum with a new lot. Increase initial cell inoculums. Lastly, adapt cells sequentially to new medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.