Sf9 Cells in Sf-900™ II SFM

Gibco™ Sf9 cells are commonly used to isolate and propagate recombinant baculoviral stocks and to produce recombinant proteins. Gibco™ Sf9 cells are adapted to serum-free suspension culture in Gibco™ Sf-900™ II SFM, which saves significant time and expense associated with the adaptation of cultures.

Gibco™ Sf9 cells (frozen in Gibco™ Sf-900™ II SFM) feature:

• Recombinant protein expression from a variety of expression systems
• Good growth in adherent or suspension culture
• Small, regular size that generates even monolayers and plaques
• Documented lineage from a low passage Master Cell Bank
• Quality and performance testing

Recombinant protein expression from a variety of expression systems

High levels of protein expression in Sf9 cells can be obtained using either the BaculoDirect™ Baculovirus Expression System, the Bac-to-Bac™ Baculovirus Expression System (Cat. No. K81001), or the InsectDirect™ Expression System (Cat. No. K81001).

Small, regular size that generates even monolayers and plaques

Gibco™ Sf9 cells generate a clean, even monolayer and plaques due to their small, round, regular size. Other cells often form more irregular monolayers and plaques.

Documented lineage from a low passage Master Cell Bank

Gibco™ Sf9 cells (in Sf-900™ II SFM) were prepared as serum-free, suspension cultures from Sf9 cells that originated at the USDA Insect Pathology Laboratory. The original Sf9 cells were cloned from the parental IPLBSF-21 (Sf21) cell line that was derived from the pupal ovarian tissue of the fall army worm, Spodoptera frugiperda. The serum-free Master Cell Banks were prepared at passage 34.

Quality and performance testing

Each lot of Gibco™ Sf9 cells is tested for cell growth and viability post-recovery from cryopreservation. In addition, the Master Seed Bank has been tested for contamination of bacteria, yeast, mycoplasma and virus and has been characterized by isozyme and karyotype analysis.

Product Use

For Research Use Only. Not for any animal or human therapeutic or diagnostic use. This product contains Dimethyl Sulfoxide (DMSO), a hazardous material. Review the Material Safety Data Sheet before handling.