MEM, autoclavable, no glutamine, powder
Invitrogen17万+抗体限时买二赠一，靶点广，灵活用！
Product Image
Gibco™

MEM, autoclavable, no glutamine, powder

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be usedRead more
Have Questions?
Catalog NumberQuantity
1170007710 L
Catalog number 11700077
Price (CNY)
523.60
飞享价
Ends: 31-Dec-2025
668.00
Save 144.40 (22%)
Each
Add to cart
Quantity:
10 L
Customize this product
Price (CNY)
523.60
飞享价
Ends: 31-Dec-2025
668.00
Save 144.40 (22%)
Each
Add to cart

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM is modified as follows:

With: Phenol Red, Earle's salts

Without: L-glutamine, HEPES, Sodium Bicarbonate

The complete formulation is available.

Gibco MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow's MEM, MEM α, DMEM, and Temin's Modification. To allow autoclaving this medium, choline chloride is substituted with choline bitartrate, phenol red is reduced, and succinic acid is added to prevent precipitation at low pH. This MEM formulation contains Earle's salts for use in a CO2 incubator. This product is made with Earle's salts.

MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.

This autoclavable form of Gibco™ cell culture medium requires pH adjustment to 4.1–4.2 before autoclaving, following by sodium bicarbonate and L-glutamine supplementation and final pH adjustment to 7.2–7.4 (see protocol for details).

Please note that the shelf life on delivered products varies. This product has a minimum shelf life of 6 months at time of shipment. For more information regarding product shelf life, including details on specific product lots, please contact Customer Service.

Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. For in vitro diagnostic use.
Specifications
Cell LineHeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Cell TypePrimary Rat Astrocytes
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™
Product TypeMEM (Minimum Essential Medium)
Quantity10 L
Shelf Life36 Months From Date of Manufacture
Shipping ConditionRoom Temperature
ClassificationAnimal Origin-free
FormPowder
Serum LevelStandard Serum Supplementation
SterilitySterile-filtered
With AdditivesPhenol Red, Earle's Balanced Salt Solution (EBSS)
Without AdditivesNo HEPES, No Sodium Bicarbonate
Unit SizeEach
Contents & Storage
Storage conditions: 2-8°C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture

Frequently asked questions (FAQs)

Where can I find the osmolality for MEM Medium?

The osmolality is listed in the COA for the particular lot number of the medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the shelf life of my powdered media once I reconstitute it?

Expiration date of most Gibco reconstituted dry format media (AGT or DPM) has not been established; end users should assess performance and stability of this reconstituted media in a system that is relevant to their process. A dry format product (either DPM or AGT) will age upon storage and while we may not be able to detect which component(s) degrade since we can't measure every component, that doesn't mean that the reconstituted liquid will have the same stability as a fresh liquid made by direct weigh methods.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all MEM, autoclavable, no glutamine, powder FAQs