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Platinum™ Quantitative RT-PCR ThermoScript™ One-Step System
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Citations & References (1)
Invitrogen™

Platinum™ Quantitative RT-PCR ThermoScript™ One-Step System

Alternative Product: Try TaqMan Fast Virus 1-Step Master Mix, our high performance, 1-step RT-qPCR master mix. With TaqMan Fast VirusRead more
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Catalog NumberNo. of Reactions
11731015100 Reactions
11731023500 Reactions
Catalog number 11731015
Price (CNY)
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No. of Reactions:
100 Reactions
Alternative Product: Try TaqMan Fast Virus 1-Step Master Mix, our high performance, 1-step RT-qPCR master mix. With TaqMan Fast Virus 1-Step Master Mix, we’ve improved on the best features of the Platinum Quantitative RT-PCR ThermoScript One-Step System and added additional convenience and performance to your 1-step gene expression analysis and RNA virus detection workflows.
Platinum Quantitative RT-PCR ThermoScript One-Step System is for real-time quantitation of RNA molecules from total or poly(A)+ RNA in a single step.

This kit provides:
• Platinum™ Taq DNA Polymerase
• ThermoScript RT polymerase
• A vial of 50 mM MgSO4

For Research Use Only. Not for use in diagnostic procedures.
Specifications
No. of Reactions100 Reactions
PolymeraseTaq DNA Polymerase
Product LinePlatinum, ThermoScript
Product TypeRT-PCR One-Step System
Quantity100 reactions
Sample TypeRNA
Shipping ConditionDry Ice
Sufficient For100 Reactions
Detection MethodPrimer-probe
PCR Method1-step RT-qPCR
Reaction SpeedStandard
Unit SizeEach
Contents & Storage
Platinum™ Quantitative RT-PCR ThermoScript™ One-Step System contains ThermoScript™ RT/Platinum™ Taq mix, and 2X ThermoScript™ reaction mix (a buffer containing 0.4 mM of each dNTP, 6 mM MgSO4 ). A vial of 50 mM MgSO4 is also provided. Store at -20°C. Guaranteed stable for 6 months when properly stored.

Frequently asked questions (FAQs)

What can I do to improve the sensitivity of my qPCR assay?

If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:

- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)

How do I set the baseline for my qPCR experiment?

Most times your instrument software can automatically set a proper baseline for your data. Check out our short video, Understanding Baselines, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=5BjFAJHW-bE).

How do I set the threshold for my qPCR experiment?

In most cases your instrument software can automatically set a proper threshold for your data. Check out our short video, Understanding Thresholds, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=H_xsuRQIM9M).

I am not getting any amplification with my TaqMan Assay or SYBR Green primer set. What is causing this?

There could be several reasons for no amplification from an assay or primer set. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/no-amplification.html) for more details.

I am getting amplification in my no-template control (NTC) wells in my qPCR experiment. What is causing this?

There could be several reasons for amplification in a NTC well. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/amplification-no-template-control.html) for more details.

View all Platinum™ Quantitative RT-PCR ThermoScript™ One-Step System, 100 reactions FAQs

Citations & References (1)

Citations & References
Abstract
Peroxisome Proliferator-activated Receptor gamma Agonists Inhibit HIV-1 Replication in Macrophages by Transcriptional and Post-transcriptional Effects.
Authors: Hayes Michael M; Lane Brian R; King Steven R; Markovitz David M; Coffey Michael J;
Journal:J Biol Chem
PubMed ID:11847231
Previous studies have demonstrated that cyclopentenone prostaglandins (cyPG) inhibit human immunodeficiency virus type 1 (HIV-1) replication in various cell types. We investigated the role of PG in the replication of HIV-1 in primary macrophages. The cyPG, PGA(1) and PGA(2), inhibited HIV-1 replication in acutely infected human monocyte-derived macrophages (MDM). Because ... More