SuperScript™ III Platinum™ One-Step qRT-PCR Kit w/ROX
Invitrogen™
SuperScript™ III Platinum™ One-Step qRT-PCR Kit w/ROX
The SuperScript III Platinum One-Step qRT-PCR Kit’s proven formula delivers reliable, accurate target detection when it matters most. The convenientRead more
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Catalog Number
No. of Reactions
11745500
500 Reactions
11745100
100 Reactions
2 Options
Catalog number 11745500
Price (CNY)
22,916.00
Each
Add to cart
No. of Reactions:
500 Reactions
Price (CNY)
22,916.00
Each
Add to cart
The SuperScript III Platinum One-Step qRT-PCR Kit’s proven formula delivers reliable, accurate target detection when it matters most. The convenient one-step format and consistent performance make it ideal for a number of gene expression applications, including infectious disease surveillance. The kit has been validated by several global public health organizations and has long been the choice of the US CDC for infectious disease surveillance.
Features of the SuperScript III Platinum One-Step qRT-PCR Kit include: • SuperScript III RT for higher temperature cDNA synthesis when addressing difficult RNA secondary structure • Platinum Taq DNA Polymerase with hot-start technology for improved specificity • LUX fluorogenic primers and dual-labeled fluorogenic probes for effective detection performance • ROX reference dye pre-mixed at optimal concentration for Applied Biosystems instruments • Validated performance on multiple real-time instrument platforms, including rotor-based systems
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Equipment)7000 System, 7300 System, 7700 System, 7900HT System
No. of Reactions500 Reactions
PolymerasePlatinum Taq DNA Polymerase
Product LinePlatinum™, SuperScript™
Product TypeOne-Step qRT-PCR Kit
Quantity500 reactions
Sample TypeRNA
Shipping ConditionDry Ice
Sufficient For500 Reactions
Detection MethodPrimer-probe
For Use With (Application)Gene Expression, Pathogen Detection, Virus Detection
PCR Method1-step RT-qPCR
Reaction SpeedStandard
Unit SizeEach
Contents & Storage
• 500 μL SuperScript™ III/Platinum™ Taq Mix • 12.5 mL 2X Reaction Mix with ROX • 2 × 1 mL Magnesium Sulfate (50 mM)
Store components in the dark at -20°C.
Frequently asked questions (FAQs)
What can I do to improve the sensitivity of my qPCR assay?
If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:
- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)
How do I set the baseline for my qPCR experiment?
Most times your instrument software can automatically set a proper baseline for your data. Check out our short video, Understanding Baselines, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=5BjFAJHW-bE).
How do I set the threshold for my qPCR experiment?
In most cases your instrument software can automatically set a proper threshold for your data. Check out our short video, Understanding Thresholds, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=H_xsuRQIM9M).
I am not getting any amplification with my TaqMan Assay or SYBR Green primer set. What is causing this?
There could be several reasons for no amplification from an assay or primer set. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/no-amplification.html) for more details.
I am getting amplification in my no-template control (NTC) wells in my qPCR experiment. What is causing this?
There could be several reasons for amplification in a NTC well. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/amplification-no-template-control.html) for more details.