SuperScript™ III Platinum™ SYBR™ Green One-Step qPCR Kit w/ROX
Invitrogen™
SuperScript™ III Platinum™ SYBR™ Green One-Step qPCR Kit w/ROX
Our One-Step qRT-PCR Kits with ROX combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise andRead more
Have Questions?
Change view
Catalog Number
No. of Reactions
11746500
500 Reactions
11746100
100 Reactions
2 Options
Catalog number 11746500
Price (CNY)
20,299.00
Each
Add to cart
No. of Reactions:
500 Reactions
Price (CNY)
20,299.00
Each
Add to cart
Our One-Step qRT-PCR Kits with ROX combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient, high-throughput one-step format. One-Step qRT-PCR kits with ROX include:
–SuperScript™ III Reverse Transcriptase: produces higher yields of full-length cDNA for broader gene representation
–Platinum™ Taq DNA Polymerase: activates faster and more completely than other hot-start enzymes to deliver more sensitive amplification (Figure 1) –ROX reference dye premixed at optimal concentration for ABI instruments
Two kit configurations allow your choice of detection methods:
–SuperScript™ III Platinum™ SYBR™ Green One-Step qRT-PCR Kit provides a highly efficient, cost-effective qRT-PCR system with SYBR Green I detection –SuperScript™ III Platinum™ One-Step qRT-PCR Kit provides sensitive, specific detection for use with LUX™ fluorogenic primers or dual-labeled probes (e.g. TaqMan™) (Figure 2)
Contents and Storage: SuperScript™ III Platinum™ SYBR™ GreenOne-Step qRT-PCR Kit with ROX includes SuperScript™ III RT/Platinum™ Taq Mix, 2X Reaction Mix (containing SYBR™ Green I, ROX Reference Dye, dNTPs, and MgSO4), and 50 mM MgSO4. SuperScript™ III Platinum™One-Step qRT-PCR Kit with ROX includes SuperScript™ III RT/Platinum™ Taq Mix, 2X Reaction Mix (containing ROX Reference Dye, dNTPs and MgSO4), and 50 mM MgSO4. Store all components at -20°C. Store ROX Reference dye in the dark. Guaranteed stable for 6 months when properly stored.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Equipment)7000 System, 7300 System, 7700 System, 7900HT System
No. of Reactions500 Reactions
PolymeraseTaq DNA Polymerase
Product LinePlatinum™, SYBR™, SuperScript™
Product TypeReal Time PCR SYBR Master Mix
Quantity500 reactions
Sample TypeRNA
Shipping ConditionDry Ice
Sufficient For500 Reactions
Concentration2X
Detection MethodSYBR
For Use With (Application)Gene Expression
PCR Method1-step RT-qPCR
Reaction SpeedStandard
Unit SizeEach
Contents & Storage
Store in freezer (-5 to -30°C).
Frequently asked questions (FAQs)
What can I do to improve the sensitivity of my qPCR assay?
If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:
- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)
How do I set the baseline for my qPCR experiment?
Most times your instrument software can automatically set a proper baseline for your data. Check out our short video, Understanding Baselines, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=5BjFAJHW-bE).
How do I set the threshold for my qPCR experiment?
In most cases your instrument software can automatically set a proper threshold for your data. Check out our short video, Understanding Thresholds, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=H_xsuRQIM9M).
I am not getting any amplification with my TaqMan Assay or SYBR Green primer set. What is causing this?
There could be several reasons for no amplification from an assay or primer set. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/no-amplification.html) for more details.
I am getting amplification in my no-template control (NTC) wells in my qPCR experiment. What is causing this?
There could be several reasons for amplification in a NTC well. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/amplification-no-template-control.html) for more details.