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EXPRESS One-Step Superscript™ qRT-PCR Kit, with premixed ROX
EXPRESS One-Step SuperScript qRT-PCR kits incorporate proven Invitrogen enzymes specifically tailored to deliver superior results on high-throughput, fast-cycling instruments forRead more
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| Catalog Number | No. of Reactions |
|---|---|
| 11791200 | 500 Reactions |
| 1179101K | 2500 Reactions |
Catalog number 11791200
Price (CNY)
15,440.00
Each
No. of Reactions:
500 Reactions
Price (CNY)
15,440.00
Each
EXPRESS One-Step SuperScript qRT-PCR kits incorporate proven Invitrogen enzymes specifically tailored to deliver superior results on high-throughput, fast-cycling instruments for your probe-based qPCR experiments. This fast, one-step mix is designed to work with a variety of instruments’ default protocols, including Applied Biosystems and non-Applied Biosystem (see universal version) qPCR platforms.
Features of the EXPRESS One-Step Superscript qRT-PCR Kit include:
• Fast-activating, antibody-mediated Platinum Taq DNA Polymerase–complete, rapid activation; provides high sensitivity and reproducibility
• The One-Step SuperScript III Reverse Transcriptase module– a unique formulation to ensure high yield and less PCR inhibition
• Flexible formats with ROX premixed or separate–designed to work with default instrument protocols on a wide range of high-throughput fast instruments (e.g., AB 7500 Fast, AB 7900 HT Fast, AB StepOne, Roche LightCycler™ 480, Corbett Rotor-Gene™)
• Contains heat-labile UDG–significantly reduces carryover contamination risk with excellent activity from room temperature to approximately 40°C. Due to the thermostability of SuperScript III, the cDNA synthesis step is run at 50°C, at which temperature the heat-labile UDG is totally and irreversibly inactivated
EXPRESS kits work with manufacturer’s default protocols on fast instruments and come in a convenient, stable SuperMix format for ultimate convenience. The kits are offered with either pre-mixed ROX or a separate tube of ROX to fit your instrument needs, as well as SYBR Green chemistry options:
EXPRESS One-Step Superscript qRT-PCR Kit, with premixed ROX (this page)
EXPRESS One-Step Superscript qRT-PCR Kit, universal
EXPRESS One-Step SYBR GreenER Kit, with premixed ROX
EXPRESS One-Step SYBR GreenER Kit, universal
Features of the EXPRESS One-Step Superscript qRT-PCR Kit include:
• Fast-activating, antibody-mediated Platinum Taq DNA Polymerase–complete, rapid activation; provides high sensitivity and reproducibility
• The One-Step SuperScript III Reverse Transcriptase module– a unique formulation to ensure high yield and less PCR inhibition
• Flexible formats with ROX premixed or separate–designed to work with default instrument protocols on a wide range of high-throughput fast instruments (e.g., AB 7500 Fast, AB 7900 HT Fast, AB StepOne, Roche LightCycler™ 480, Corbett Rotor-Gene™)
• Contains heat-labile UDG–significantly reduces carryover contamination risk with excellent activity from room temperature to approximately 40°C. Due to the thermostability of SuperScript III, the cDNA synthesis step is run at 50°C, at which temperature the heat-labile UDG is totally and irreversibly inactivated
EXPRESS kits work with manufacturer’s default protocols on fast instruments and come in a convenient, stable SuperMix format for ultimate convenience. The kits are offered with either pre-mixed ROX or a separate tube of ROX to fit your instrument needs, as well as SYBR Green chemistry options:
EXPRESS One-Step Superscript qRT-PCR Kit, with premixed ROX (this page)
EXPRESS One-Step Superscript qRT-PCR Kit, universal
EXPRESS One-Step SYBR GreenER Kit, with premixed ROX
EXPRESS One-Step SYBR GreenER Kit, universal
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Equipment)7000 System, 7300 System, 7700 System, 7900HT Fast System, 7900HT System, Eppendorf™ MasterCycler™ RealPlex, StepOne™, Fast Mode, StepOne™, Standard Mode
FormatTube
No. of Reactions500 Reactions
PolymeraseTaq DNA Polymerase
Product LineSuperScript™
Product TypeqRT-PCR Kit
Quantity500 reactions
Sample TypeRNA
Sufficient For500 Reactions
Detection MethodPrimer-probe
For Use With (Application)Gene Expression
PCR Method1-step RT-qPCR
Reaction SpeedFast or Standard
Unit SizeEach
Contents & Storage
EXPRESS One-Step SuperScript™ qRT-PCR SuperMixes are composed of 2 modules: an EXPRESS RT module (containing SuperScript™ III Reverse Transcriptase and RNAseOUT™) and an EXPRESS qPCR SuperMix module with ROX premixed or in a separate tube.
Store at -20°C upon receipt. Once thawed, the EXPRESS qPCR SuperMix can be stored for up to 1 month at +4·C. Product is guaranteed stable for 6 months when properly stored.
Store at -20°C upon receipt. Once thawed, the EXPRESS qPCR SuperMix can be stored for up to 1 month at +4·C. Product is guaranteed stable for 6 months when properly stored.