PFHM-II Protein-Free Hybridoma Medium (liquid)
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Gibco™

PFHM-II Protein-Free Hybridoma Medium (liquid)

Gibco® Protein Free Hybridoma Medium II (PFHM II) is an animal origin-free, serum-free, protein-free medium specifically developed for the abilityRead more
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Catalog NumberQuantity
120400771000 mL
Catalog number 12040077
Price (CNY)
2,028.00
Each
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Quantity:
1000 mL
Customize this product
Price (CNY)
2,028.00
Each
Add to cart
Gibco® Protein Free Hybridoma Medium II (PFHM II) is an animal origin-free, serum-free, protein-free medium specifically developed for the ability to support the growth of hybridomas for antibody production.

Gibco® PFHM II Features:

• Ability to support suspension and stationary hybridomas and myelomas
• Complete, ready-to-use formulation
• Animal origin-free, serum-free, protein-free formulation

Ability to support suspension and stationary hybridomas and myelomas
Suitable for the culture of recombinant myeloma lines as well as traditional hybridomas, Gibco® PFHM II provides high cell densities and IgG production. Cholesterol-dependent cultures such as NS0 require the addition of 250X Cholesterol Lipid Concentrate.

Complete, ready-to-use formulation
Gibco® PFHM II is a complete medium that is ready-to-use. No supplementation is required.

Animal origin-free, serum-free, protein-free formulation
Gibco® PFHM II is an animal origin-free, serum-free, protein-free formulation, allowing for easier purification of your protein of interest.

Product Use
For Research Use Only. Not for use in diagnostic procedures.

cGMP Manufacturing and Quality System
Gibco® PFHM II is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable Gibco® PFHM II product made in our Scotland facility (12040-051). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeHybridomas
Product LineGibco™
Product TypeHybridoma Medium
Quantity1000 mL
Shelf Life12 Months
Shipping ConditionRoom Temperature
ClassificationProtein-free
Culture TypeSuspension Cell Culture
FormLiquid
Serum LevelSerum-free
With AdditivesGlutamine, Phenol Red
Unit SizeEach
Contents & Storage
Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Frequently asked questions (FAQs)

How do I adapt my cells to serum-free medium?

Cells can be adapted by Sequential or Direct Adaptation. Suggested protocols for each are below, and you can also find more information by searching "Adaptation of Cell Cultures to a Serum-Free Medium" from our website home page.

SEQUENTIAL ADAPTATION
1) Subculture the cells growing in serum-supplemented medium into a 25%:75% mixture of SFM and serum supplemented medium.
2) When the cell density is 5 x 10E5 cells/ml, subculture the cells into a 50%:50% mixture of SFM and serum supplemented medium at a cell density 2.5 x 10E5 to 3 x 10E5 cells/ml.
3) Continue to subculture after the cell density 5 x 10E5 cells/ml in gradually increasing proportions of SFM until the serum is ~0.1% with about 85% cell viability.
4) Subculture the cells into SFM with an innoculum of 2.5 x 10E5 to 3 x 10E5 cells/ml.
5) When the cell density is 1 x 10E6 to 3 x 10E6 cells/ml (4 to 6 days post planting) subculture the cells again.
6) Stock cultures of SFM adapted cells should be subcultured in SFM every 3 to 5 days when the cell density is 1 x 10E6 to 3 x 10E6 cells/ml with 90% viability.

DIRECT ADAPTATION
Some cells can be directly adapted from serum-containing medium to SFM. For direct adaptation, the cell innoculum should be 1.5 x 10E5 to 3 x 10E5 cells/ml.
Cells should be subcultured when the cell density is 1 x 10E6 to 3 x 10E6 cells/ml. Cells are fully adapted to SFM when the cell density is 2 x 10E6 to 4 x 10E6 cells/ml after 4 to 7 days in culture.
Stock cultures of cells adapted to SFM should be subcultured in SFM every 3 to 5 days when the cell density is 1 x 10E6 to 3 x 10E6 cells/ml with 90% viability.

Why is it necessary to gradually adapt the cells to serum-free medium?

Some cells, such as insect cells, are sensitive to changes in their medium. By sequentially adapting cells, the medium is changed with minimal effects on cell growth.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I freeze PFHM-II Protein-Free Hybridoma Medium (liquid) (Cat. No. 12040077)?

We do not recommend freezing our liquid media products. Freezing can cause some components like amino acids and inorganic salts to precipitate upon thawing.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.