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Invitrogen™
Platinum™ SuperFi™ DNA Polymerase
Invitrogen Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR.
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| Catalog Number | No. of Reactions |
|---|---|
| 12351250 | 5 x 500 Reactions |
| 12351010 | 100 Reactions |
| 12351050 | 500 Reactions |
Catalog number 12351250
Price (CNY)
39,603.00
Each
No. of Reactions:
5 x 500 Reactions
Price (CNY)
39,603.00
Each
Invitrogen Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR. Featuring >300X Taq fidelity, Platinum SuperFi DNA Polymerase is ideally suited for cloning, mutagenesis, and other applications benefiting from supreme sequence accuracy.
Platinum SuperFi DNA Polymerase is engineered with a DNA-binding domain resulting in high processivity and increased resistance to PCR inhibitors. This feature also enables fast-cycling protocols and amplification of long targets. The Platinum hot-start technology is based on proprietary antibodies that inhibit enzyme activity until the initial PCR denaturation step, preventing non-specific amplification and primer degradation.
Benefits of Platinum SuperFi DNA Polymerase
- Exceptional >300X Taq fidelity
- High specificity and increased yields with Platinum hot-start technology
- Robust amplification of difficult targets including those of sub-optimal purity or high GC content
- Room temperature reaction setup and 24 hour bench top stability of pre-assembled reactions
- Amplification of samples with suboptimal purity
Applications
- High-fidelity PCR
- Cloning and sub-cloning
- Site-directed mutagenesis
- Amplification of GC-rich templates
- Template generation for sequencing
- High-throughput PCR
- Long PCR
Notes
- Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Fidelity (vs. Taq)300X
Hot StartBuilt-In Hot Start
No. of Reactions5 x 500 Reactions
OverhangBlunt
PolymerasePlatinum SuperFi DNA Polymerase
Quantity5 x 500 units
Reaction FormatSeparate Components
Shipping ConditionWet or Dry Ice
Size (Final Product)20 kb or less
For Use With (Application)Hot-start PCR, High-fidelity PCR
GC-Rich PCR PerformanceHigh
Reaction SpeedFast
Unit SizeEach
Contents & Storage
5 boxes of Platinum SuperFi Master Mix, each containing:
• Platinum SuperFi DNA Polymerase (250 μL at 2 U/μL)
• 5X SuperFi Buffer (6 x 1.25 mL)
• 5X SuperFi GC Enhancer (4 x 1.25 mL)
Store at –5°C to –30°C.
• Platinum SuperFi DNA Polymerase (250 μL at 2 U/μL)
• 5X SuperFi Buffer (6 x 1.25 mL)
• 5X SuperFi GC Enhancer (4 x 1.25 mL)
Store at –5°C to –30°C.