PCR Cloning System with Gateway™ Technology with pDONR™221 & OmniMAX™2 Competent Cells
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Citations & References (2)
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PCR Cloning System with Gateway™ Technology with pDONR™221 & OmniMAX™2 Competent Cells

Gateway™ Technology enables rapid cloning of one or more genes into virtually any protein expression system. Once you have anRead more
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Catalog NumberQuantity
1253502920 Reactions
Catalog number 12535029
Price (CNY)
5,856.00
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Ends: 31-Dec-2025
7,508.00
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Quantity:
20 Reactions
Price (CNY)
5,856.00
飞享价
Ends: 31-Dec-2025
7,508.00
Save 1,652.00 (22%)
Each
Add to cart
Gateway™ Technology enables rapid cloning of one or more genes into virtually any protein expression system. Once you have an entry clone, you can recombine your gene of interest into a variety of expression vectors adapted for use in Gateway™ Technology. The PCR Cloning System with Gateway™ Technology is designed to create an entry clone following PCR with attB-modified custom primers (Figure 1). The PCR product is directionally cloned with efficiencies typically >99%. The PCR Cloning System with Gateway™ Technology offers:

• Rapid, efficient, directional cloning of PCR products
• Versatility to use any amplification enzyme
• Cloning without the need for restriction endonucleases or ligase
• Selection of entry clones with kanamycin or Zeocin™

In addition, the pDONR™221 and pDONR™/Zeo vectors have a pUC origin for high plasmid yields and universal M13 sequencing sites for ease of use.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cloning MethodGateway™
No. of Reactions20 reactions
Product LineOne Shot
Product TypePCR Cloning System
Quantity20 Reactions
VectorpDONR
Unit SizeEach
Contents & Storage
The PCR Cloning Kit with Gateway™ Technology includes Gateway™ BP Clonase™ II enzyme mix and reagents, Gateway™ pDONR™221 or pDONR™/Zeo vector, M13 sequencing primers, PEG/Mg2+ solution, proteinase K, pEXP7-tet positive control, and One Shot™ OmniMAX™2 Competent Cells. Store competent cells and enzyme mix at -80°C. Store all other components at -20°C.

Frequently asked questions (FAQs)

Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?

In the single-step protocol for the BP/LR Clonase reaction, we would not recommend substituting the BP Clonase II/LR Clonase II enzymes with BP Clonase /LR Clonase enzymes as this would result in very low recombination efficiency.

Do you have a recommended single-step protocol for BP/LR recombination?

Yes, we have come up with a single-step protocol for BP/LR Clonase reaction (http://www.thermofisher.com/us/en/home/life-science/cloning/gateway-cloning.html#1), where DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money.

How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?

We would recommend performing a BP reaction with a Donor vector in order to obtain an entry clone. This entry clone can then be used in an LR reaction with the Destination vector to obtain the new expression clone.

Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?

We do not offer the 5X LR Clonase buffer and 5X BP Clonase buffer as standalone products. They are available as part of the enzyme kits.

Do you offer Gateway vectors for expression in plants?

We do not offer any Gateway vectors for expression in plants.

View all PCR Cloning System with Gateway™ Technology with pDONR™221 & OmniMAX™2 Competent Cells FAQs

Citations & References (2)

Citations & References
Abstract
Participation of an Endomembrane Cation/H+ Exchanger AtCHX20 in Osmoregulation of Guard Cells.
Authors:Padmanaban S, Chanroj S, Kwak JM, Li X, Ward JM, Sze H,
Journal:Plant Physiol
PubMed ID:17337534
'Guard cell movement is induced by environmental and hormonal signals that cause changes in turgor through changes in uptake or release of solutes and water. Several transporters mediating these fluxes at the plasma membrane have been characterized, however less is known about transport at endomembranes. CHX20, a member of a ... More
Cytosolic Hydroxymethyldihydropterin Pyrophosphokinase/Dihydropteroate Synthase from Arabidopsis thaliana: A SPECIFIC ROLE IN EARLY DEVELOPMENT AND STRESS RESPONSE.
Authors:Storozhenko S, Navarrete O, Ravanel S, De Brouwer V, Chaerle P, Zhang GF, Bastien O, Lambert W, Rébeillé F, Van Der Straeten D,
Journal:J Biol Chem
PubMed ID:17289662
In plants, 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase/7,8-dihydropteroate synthase (mitHPPK/DHPS) is a bifunctional mitochondrial enzyme, which catalyzes the first two consecutive steps of tetrahydrofolate biosynthesis. Mining the Arabidopsis genome data base has revealed a second gene encoding a protein that lacks a potential transit peptide, suggesting a cytosolic localization of the isoenzyme (cytHPPK/DHPS). When ... More