BGJb Medium
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BGJb Medium
Gibco™

BGJb Medium

BGJb medium was developed for the growth of fetal rat long bones in a chemically defined medium. BGJb is aRead more
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Catalog NumberQuantity
12591038500 mL
Catalog number 12591038
Price (CNY)
1,178.80
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Ends: 31-Dec-2025
1,504.00
Save 325.20 (22%)
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Quantity:
500 mL
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Price (CNY)
1,178.80
Online Exclusive
Ends: 31-Dec-2025
1,504.00
Save 325.20 (22%)
Each
Add to cart

BGJb medium was developed for the growth of fetal rat long bones in a chemically defined medium. BGJb is a medium modified by Sylvia Fitton-Jackson from the original BGJ medium developed by Biggers, Gwatkin, and Judah. BGJb medium creates favorable conditions for growth and calcification of fetal bones.

This BGJb medium is manufactured as follows:
WithWithout
• L-glutamine• HEPES
• Phenol Red 


The complete formulation is available.

Gibco™ BGJb medium contains higher levels of amino acids and glucose, and an increased buffering capacity to improve the conditions for growth and calcification of fetal bone.

Using BGJb medium
BGJb contains no proteins, lipids, or growth factors. Therefore, BGJb requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). BGJb uses a sodium bicarbonate buffer system (3.5 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

For Research Use Only. Not for use in diagnostic procedures. Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law. For in vitro diagnostic use.
Specifications
Cell TypeFetal Bones
Concentration1 X
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco
Product TypeBGJb Medium
Quantity500 mL
Shelf Life12 Months From Date of Manufacture
Shipping ConditionRoom Temperature
ClassificationAnimal Origin-free
FormLiquid
SterilitySterile-filtered
With AdditivesHigh Glucose, Glutamine, Phenol Red
Without AdditivesNo HEPES, No Sodium Pyruvate
Unit SizeEach
Contents & Storage
Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Frequently asked questions (FAQs)

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I see a decrease in growth of my culture. What should I do?

Try changing the medium or serum. Compare media formulations for differences in glucose, amino acids, and other components. Compare an old lot of serum with a new lot. Increase initial cell inoculums. Lastly, adapt cells sequentially to new medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

My cells are not adhering to the culture vessel. What should I do?

This can occur if cells are overly trypsinized. Trypsinize for a shorter time or use less trypsin. Mycoplasma contamination could also cause this problem. Segregate your culture and test for mycoplasma infection. Lastly, check for attachment factors in the medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all BGJb Medium FAQs