HBSS (10X), no calcium, no magnesium, phenol red
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Gibco™

HBSS (10X), no calcium, no magnesium, phenol red

Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation,Read more
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Catalog NumberQuantity
14180046
also known as 14180-046
500 mL
Catalog number 14180046
also known as 14180-046
Price (CNY)
-
Quantity:
500 mL
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Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Formulations without calcium and magnesium are required for rinsing chelators from the culture before cell dissociation. We offer a variety of Gibco™ HBSS formulations for a range of cell culture applications. Find the right formulation using the media selector tool.

This HBSS is modified as follows:

  • With: Glucose, Phenol Red
  • Without: Calcium, Magnesium, Sodium Bicarbonate

The complete formulation is available.

Concentrated forms of Gibco™ HBSS require pH adjustment and filtration at the time of preparation (see protocol for details).

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Chemical Name or MaterialHanks' Balanced Salt Solution (HBSS)
IncludesNo Calcium, No Magnesium
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Osmolality2600–2900 mOsm/kg
Recommended StorageStorage: 15°C to 30° C
Shipping: Ambient
Shelf life: 36 months from date of manufacture
SterilitySterile-filtered
Concentration10X
Physical FormLiquid
Product LineGibco™
Quantity500 mL
Solution TypeHank's Balanced Salt
pH5.6 to 6.1
Unit SizeEach

Frequently asked questions (FAQs)

Can I store Hank's Balanced Salt Solution (HBSS) in the refrigerator?

The recommended storage condition for our HBSS products is at 15-30 degrees C. Generally, storage at 4 degrees C will not impact these products. The only concern would be for the 10x HBSS solutions with calcium and magnesium as these salts might precipitate when stored outside the recommended temperature ranges. As long as you don't see any precipitation upon refrigeration, the product will be fine.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you offer media without phenol red (phenol red-free media)?

Yes, please visit this page for a list of our phenol red-free media (http://tools.thermofisher.com/content/sfs/brochures/Phenol_RedFree_Media_Glance.pdf).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is it true that phenol red has estrogenic properties?

Phenol red in tissue culture is a weak estrogen. Implications concerning study of estrogen-responsive cells in culture are discussed in Proc Natl Acad Sci U S A 83:2496 (1986).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.