DPBS, no calcium, no magnesium
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DPBS, no calcium, no magnesium
Gibco™

DPBS, no calcium, no magnesium

Dulbecco's phosphate-buffered saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washingRead more
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Catalog NumberQuantityPackaging
1419025010 x 500 mLBottle
14190144500 mLBottle
141901361000 mLBottle
141902356 x 1000 mLBottle
141903425 LBag
1419035910 LBag
1419036720 LBag
Catalog number 14190250
Price (CNY)
2,815.20
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Ends: 31-Dec-2025
3,589.00
Save 773.80 (22%)
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10 x 500 mL
Packaging:
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Price (CNY)
2,815.20
飞享价
Ends: 31-Dec-2025
3,589.00
Save 773.80 (22%)
Each
Add to cart

Dulbecco's phosphate-buffered saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. Formulations without calcium and magnesium are required for rinsing chelators from the culture before cell dissociation.

We offer a variety of Gibco™ DPBS formulations for a range of cell culture applications.

This DPBS is modified as follows:

Without: Calcium, Magnesium, Phenol Red

The complete formulation is available.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Chemical Name or MaterialDPBS
ColorClear
IncludesNo Sodium Pyruvate
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Osmolality270 - 300 mOsm/kg
PackagingBottle
Recommended StorageStorage conditions: 15°C to 30°C
Shipping conditions: Room temperature
Shelf life: 36 months from date of manufacture
SterilitySterile-filtered
Concentration1 X
For Use With (Application)Mammalian Cell Culture
Physical FormLiquid
Product LineGibco™
Quantity10 x 500 mL
Solution TypeDulbecco's Phosphate Buffered Saline
pH7.0 to 7.3
Unit SizeEach

Frequently asked questions (FAQs)

What is the difference between DPBS, no calcium, no magnesium, 10 x 500 mL, Cat. Nos. 14190250 and 1490169?

DPBS, no calcium, no magnesium, 10x 500 mL (Cat. Nos. 14190250 and 14190169) have the same formulation. The only difference is in the manufacturing site.

The DPBS, no calcium, no magnesium (Cat. no. 14190169) is manufactured in our Inchinnan, Scotland facility and is intended for sale in Europe, Africa, and the Middle East—higher freight charges will apply to orders shipped elsewhere. DPBS, no calcium, no magnesium (Cat. No. 14190250) is manufactured in Grand Island, US, and is recommended for our customers in North America, Latin America, and the Asia/Pacific region.

The complete formulation can be found here:

14190 - DPBS, no calcium, no magnesium

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the shelf life of DPBS after it has been opened?

We cannot provide an official expiration date for open bottles as this depends on the customer's use. Once opened, Thermo Fisher Scientific can no longer guarantee the performance of any product. However, if the storage conditions are followed as recommended, the stated expiration date (36 months from the date of manufacture) should apply to opened bottles as DPBS is extremely stable.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the shelf life of DPBS (Cat. No. 14190136)?

DPBS has a shelf life of 36 months from the date of manufacture, when stored as recommended (15-30 degrees C).

Does Diploid Growth Serum-Reduced Medium (SRM) contain L-glutamine?

The basal Diploid Growth Serum-Reduced Medium already contains 6 mM L-glutamine.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do I have to adjust my infection parameters when using Diploid Production Serum-Free Medium (SFM)?

We recommend evaluating performance with Diploid Production Serum-Free Medium using current conditions, however, multiplicity of infection, time of infection, and time of harvest may be different than with conventional media. Closely monitor cells for cytopathic effect and evaluate viral titers at multiple time points.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all DPBS, no calcium, no magnesium, 10 x 500 mL bottle FAQs