HEPES (1M)
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HEPES (1M)
Gibco™

HEPES (1M)

Gibco HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic organic chemical buffering agent commonly used in cell culture media. It is membrane impermeable, relatively stable, and has limited interference in key processes.
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Catalog NumberQuantity
1563013020 x 100 mL
1563010620 mL
15630080100 mL
Catalog number 15630130
Price (CNY)
21,744.00
Each
Add to cart
Quantity:
20 x 100 mL
Price (CNY)
21,744.00
Each
Add to cart

Gibco HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic organic chemical buffering agent commonly used in cell culture media. The addition of 10–25 mM HEPES provides extra buffering capacity when cell culture requires extended periods of manipulation outside of a CO2 incubator.

Gibco HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a good buffering choice for many cell culture systems because it:

  • Is membrane impermeable
  • Has limited effect on biochemical reactions
  • Is chemically and enzymatically stable
  • Has a very low visible and UV light absorbance

cGMP manufacturing and quality system

For supply chain continuity, we manufacture this product at two separate facilities located in Grand Island, NY, and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements and are certified to the ISO 13485 standard.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Chemical Name or MaterialZwitterionic Organic Chemical Buffer
Recommended StorageStorage conditions: 2°C to 8°C
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture
SterilitySterile
Sterilization MethodSterile-filtered
For Use With (Application)Chromatin Biology
GradeBiochemical
Physical FormLiquid
Quantity20 x 100 mL
Solution TypeBuffer
Unit SizeEach

Frequently asked questions (FAQs)

My Gibco HEPES (1M) (Cat. No. 15630080) was accidentally frozen. Can I still use it?

This product should be stored at 2-8 degrees C and should not be frozen. The biggest issue with accidentally freezing this product is its solubility. If this product was accidentally frozen, we recommend placing it in a 2-8 degree environment and allowing it to slowly thaw overnight. If the product is fully thawed and shows no signs of precipitation, then it should still be usable, but we cannot guarantee effectiveness.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is HEPES (1 M) in distilled water or in saline solution?

1M HEPES is in water with a pH range of 7.2 - 7.5.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the endotoxin specification for HEPES (1 M) (Cat. Nos. 15630080, 15630106, and 15630130)?

Sorry, we do not test endotoxin levels for this product.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.