Gentamicin (10 mg/mL)

Citations & References (6)

Gibco™

Gentamicin (10 mg/mL)

Gentamicin sulfate is a water-soluble antibiotic drug originally purified from the fungus Micromonospora purpurea. Gentamicin acts by binding to theRead more

Catalog Number	Quantity
15710064	10 mL

Catalog number 15710064

Price (CNY)

Quantity:

10 mL

Gentamicin sulfate is a water-soluble antibiotic drug originally purified from the fungus Micromonospora purpurea. Gentamicin acts by binding to the 30S subunit of the bacterial ribosome leading to inhibition protein synthesis and death in susceptible bacteria. Gibco™ Gentamicin is effective against a wide variety of gram-positive and gram-negative bacteria, and is used for the prevention of bacterial contamination on cell cultures. The recommended working concentration ranges from 0.5 to 50 μg/ml. We offer a variety of antibiotics and antimycotics for cell culture applications.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

Dual-Site cGMP Manufacturing
Gibco™ Gentamicin is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable Gibco™ Gentamicin product made in our Scotland facility (15710-049). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration10 mg/mL

Culture TypeMammalian Cell Culture

For Use With (Application)Prevention of Cell Culture Contamination

Quantity10 mL

Shelf Life24 Months

Shipping ConditionRoom Temperature

FormLiquid

Product TypeAntibiotic

SterilitySterile-filtered

Unit SizeEach

Contents & Storage

Storage conditions: 15-30°C
Shipping conditions: Ambient
Shelf life: 24 months from date of manufacture

Frequently asked questions (FAQs)

If Gentamicin (10 mg/mL) is accidentally stored at 2-8 degrees C, would it affect the stability of the antibiotic?

No, storing Gentamicin solution for several days at 2-8 degrees C will not have any negative impact on its performance or stability. However, as Gentamicin solution has been shown to be stable at room temperature, the recommended storage temperature is ~25 degrees C.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I decontaminate my cultures?

When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.

1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.

The following is a suggested procedure for determining toxicity levels and decontaminating cultures:

1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What antibiotics do you offer to help control or eliminate cell culture contamination?

Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations & References (6)

Citations & References

Abstract

Novel Cav2.1 splice variants isolated from Purkinje cells do not generate P-type Ca2+ current.

Authors: Tsunemi Taiji; Saegusa Hironao; Ishikawa Kinya; Nagayama Shin; Murakoshi Takayuki; Mizusawa Hidehiro; Tanabe Tsutomu;

Journal:J Biol Chem

PubMed ID:11756409

'The alpha(1)2.1 (alpha(1A)) subunits of P-type and Q-type Ca(2+) channels are encoded by a single gene, Cacna1a. Although these channels differ in the inactivation kinetics and sensitivity to omega-agatoxin IVA, the mechanism underlying these differences remains to be clarified. Alternative splicings of the Cacna1a transcript have been postulated to contribute ... More

Cell-autonomous impact of polysialic acid-producing enzyme ST8SIA2 on developmental migration and distribution of cortical interneurons.

Authors:Schuster UE, Rossdam C, Röckle I, Schiff M, Hildebrandt H

Journal:J Neurochem

PubMed ID:31608978

'In humans, variations in the polysialic acid-producing enzyme ST8SIA2 and disturbances in the cortical inhibitory system are associated with neurodevelopmental psychiatric disorders such as schizophrenia and autism. In mice, the ST8SIA2-dependent formation of polysialic acid during embryonic development is crucial for the establishment of interneuron populations of the medial prefrontal ... More

Identification of Novel Protein Targets of Dimethyl Fumarate Modification in Neurons and Astrocytes Reveals Actions Independent of Nrf2 Stabilization.

Authors:Piroli GG, Manuel AM, Patel T, Walla MD, Shi L, Lanci SA, Wang J, Galloway A, Ortinski PI, Smith DS, Frizzell N

Journal:Mol Cell Proteomics

PubMed ID:30587509

'The fumarate ester dimethyl fumarate (DMF) has been introduced recently as a treatment for relapsing remitting multiple sclerosis (RRMS), a chronic inflammatory condition that results in neuronal demyelination and axonal loss. DMF is known to act by depleting intracellular glutathione and modifying thiols on Keap1 protein, resulting in the stabilization ... More

Vesicular Stomatitis Virus Transcription Is Inhibited by TRIM69 in the Interferon-Induced Antiviral State.

Authors:Kueck T, Bloyet LM, Cassella E, Zang T, Schmidt F, Brusic V, Tekes G, Pornillos O, Whelan SPJ, Bieniasz PD

Journal:J Virol

PubMed ID:31578292

'Interferons (IFNs) induce the expression of interferon-stimulated genes (ISGs), many of which are responsible for the cellular antiviral state in which the replication of numerous viruses is blocked. How the majority of individual ISGs inhibit the replication of particular viruses is unknown. We conducted a loss-of-function screen to identify genes ... More

Use of Precision-Cut Lung Slices as an

Authors:Rosales Gerpe MC, van Vloten JP, Santry LA, de Jong J, Mould RC, Pelin A, Bell JC, Bridle BW, Wootton SK

Journal:Mol Ther Methods Clin Dev

PubMed ID:30112421

Organotypic slice cultures recapitulate many features of an intact organ, including cellular architecture, microenvironment, and polarity, making them an ideal tool for the

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