Platinum™ Taq DNA Polymerase, DNA-free
Platinum™ Taq DNA Polymerase, DNA-free
Invitrogen™

Platinum™ Taq DNA Polymerase, DNA-free

Invitrogen Platinum Taq DNA Polymerase, DNA-free, is manufactured using a novel, closed single-use system together with stringent quality-control testing to minimize the risk of contamination with bacterial and human DNA.
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Catalog NumberNo. of Reactions
15966005400 Reactions
Catalog number 15966005
Price (CNY)
2,902.00
Each
Add to cart
No. of Reactions:
400 Reactions
Request bulk or custom format
Price (CNY)
2,902.00
Each
Add to cart
Invitrogen Platinum Taq DNA Polymerase, DNA-free, is manufactured using a novel, closed single-use system together with stringent quality-control testing to minimize the risk of contamination with bacterial and human DNA. This enzyme is an ideal choice for PCR-based applications requiring the high sensitivity without false-positive results from reagent-borne contamination. This DNA-free enzyme provides the same high level of performance and lot-to-lot consistency as standard Platinum Taq DNA Polymerase.

Platinum Taq DNA Polymerase, DNA-free, is a recombinant Taq polymerase complexed with a proprietary antibody that blocks polymerase activity at ambient temperatures and dissociates after the initial denaturation step at 94°C. This automatic hot start provides increased sensitivity, specificity, and yield, while allowing convenient reaction assembly at room temperature. Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase, DNA-free, has a non-template–dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends and has a 5' to 3' exonuclease activity.

Features

  • Certified low DNA contamination level (≤0.01 copy of bacterial gDNA per enzyme unit)
  • Confidence in qualitative and quantitative detection of low-abundance microorganisms due to signal absence with no-template/reagents-only PCR controls
  • Same performance as Platinum Taq DNA Polymerase manufactured using conventional process

Applications

  • Amplification of DNA from complex genomic and viral templates
  • Hot start PCR
  • RT-PCR
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Fidelity (vs. Taq)1X
FormatTube
Hot StartBuilt-In Hot Start
No. of Reactions400 Reactions
Overhang3'-A
PolymerasePlatinum Taq DNA Polymerase
Product TypeTaq DNA Polymerase
Quantity500 Units
Reaction FormatSeparate Components
Shipping ConditionDry Ice
Size (Final Product)5 kb or less
Starting MaterialDNA
Concentration5 U/μL
For Use With (Application)Hot-start PCR
GC-Rich PCR PerformanceLow
Reaction SpeedStandard
Unit SizeEach
Contents & Storage
• Platinum Taq DNA Polymerase, DNA-free, 100 μL
• 10X PCR Buffer (without magnesium), DNA-free, 1.25 mL
• 50 mM MgCl2, DNA-free (1 mL)

Store at -20°C in a non-frost-free freezer.

Frequently asked questions (FAQs)

What is the unit concentration of Platinum Taq DNA Polymerase, DNA-free?

Platinum Taq DNA Polymerase, DNA-free is available in 5.0 U/µL enzyme concentration.

Can I assemble my PCR reactions with Platinum Taq DNA Polymerase, DNA-free at room temperature?

Yes. Due to stable antibody-mediated hot-start technology, Platinum Taq DNA Polymerase, DNA-free is highly stable. The PCR reactions can be assembled at room temperature without any loss of amplification specificity.

I am using Platinum Taq DNA Polymerase, DNA-free for PCR. "No-template controls" in my experiment are showing a background amplification signal. What should I do differently?

Amplification signal in “no-template controls” (NTC), also known as false-positive signal, or background amplification in “reagent-only control”, may arise from contaminating DNA that entered control reactions from the environment, from the researcher, or was introduced into the PCR via contaminated PCR reagents or consumables. To avoid contaminating DNA in your experiments, it is important to follow these recommendations:

- Be cautious to maintain a DNA-free environment during handling and opening the vials with DNA polymerase, PCR buffer, and MgCl2
- Ensure that all components of the PCR, like dNTPs, primers, probes, or water are free of contaminating DNA that can give false positive PCR results
- Avoid opening the tubes containing DNA-free reagents multiple times to minimize risk of DNA contamination
- Work in a UV-irradiated workstation
- Use certified DNA-free pipette tips and PCR consumables

Can I use the same cycling protocol that I use with standard Taq for PCR reactions with Platinum Taq DNA Polymerase, DNA-free?

PCR reactions with Platinum Taq DNA Polymerase, DNA-free can be run using the same cycling protocol as that for standard Taq.

Can I use Platinum Taq DNA Polymerase, DNA-free in qPCR?

Yes, Platinum Taq DNA Polymerase, DNA-free can be used in qPCR master mixes for target detection and quantification in a real-time PCR instrument using probes or SYBR Green dye.

View all Platinum™ Taq DNA Polymerase, DNA-free FAQs