PepMap™ 100 C8 HPLC Columns
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Thermo Scientific™

PepMap™ 100 C8 HPLC Columns

Use this nanocolumn for high-resolution analyses of tryptic, natural, and synthetic peptides.
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Catalog NumberLength (Metric)Diameter (Metric)Particle Size
161185150 mm0.075 mm3 μm
1611945 mm0.3 mm5 μm
Catalog number 161185
Price (CNY)
-
Length (Metric):
150 mm
Diameter (Metric):
0.075 mm
Particle Size:
3 μm

Choose Thermo Scientific™ Acclaim™ PepMap™ 100 C8 LC Columns when separating very hydrophobic peptides (nontryptic peptides) as an alternative to the PepMap™ C18 column.

This column provides high-resolution, high-sensitivity protein identification for proteomics and biomarker discovery and verification, and is ideal for:

 

High loading capcity

Acclaim PepMap columns are exceptionally suitable for the analysis of low abundant peptides often found in complex proteomics samples.

The PepMap 100 C8 LC Columns is available in 150mm length with a choice of 75, 300, 1000, and classic 75μm I.D. to allow tuning for throughput, sensitivity and amount of sample available.

Superior protein and peptide separations

Trifluoroacetic acid (TFA)-free LC/MS minimizes suppression effects for enhanced MS sensitivity

Outstanding separation efficiency

Superior resolution and recovery

Easy installation with Thermo Scientific™ Dionex™ nanoViper™ Fingertight Fittings

Columns are available with nanoViper Fingertight Fittings

Requires no tools

Provides virtually zero-dead-volume connections

Withstands pressures up to a 1000 bar

About Nano, capillary, and micro Acclaim PepMap columns

Nano (75μm), capillary (300μm) and micro (1000μm) columns are ideal for protein and peptide analyses. Sample amount is often limited and smaller I.D. columns can increase in sensitivity in sample-limited situations. While nano LC columns are often used in proteomics, the concentrating effects of capillary and micro columns are beneficial for metabolomics or for facilitating LC-MS interfacing. Thermo Scientific Acclaim PepMap columns are offered in nano, capillary and micro column in a variety of chemistries for peptides and protein analysis.

Specifications
Column FormatAnalytical Column
Column TypeReversed Phase
EndcappedYes
Flow Rate40 μL/min
For Use WithLC/MS, ESI-MS, MALDI-MS
Max. Pressure7250 psi (500 bar)
Packing MaterialSpherical, Fully Porous, Ultrapure Silica
Stationary PhaseC8
Surface Area450 m²/g
Temperature60°C
USP TypeL7
Carbon Load9%
Diameter (Metric)0.075 mm
Length (Metric)150 mm
MaterialStainless Steel
Particle Size3 μm
PhaseReversed Phase
Pore Size100 Å
Product LinenanoLC
TypeAnalytical Column
pH2 to 8
Unit SizeEach