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Thermo Scientific™
pTK-Green Renilla Luc Vector for Luciferase Assays
The Thermo Scientific pCMV-Green Renilla Luc vector is a derivative of pMCS-Green Renilla Luc. It contains the luciferase gene underRead more
| Catalog Number | Quantity |
|---|---|
| 16154 | 10 μg |
Catalog number 16154
Price (CNY)
1,226.00
Each
Quantity:
10 μg
Price (CNY)
1,226.00
Each
The Thermo Scientific pCMV-Green Renilla Luc vector is a derivative of pMCS-Green Renilla Luc. It contains the luciferase gene under the control of the Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter, creating a constitutive expression vector that can be used as a normalization control to account for experimental variation in combination with other reporters.
Features of the pTK-Green Renilla Luc vector:
- Weak (TK) constitutive promoter for co-transfection and normalization
- Intracellular Green Renilla luciferase gene, optimized for high expression in mammalian systems
- Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
- Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
- High-copy pUC bacterial DNA replication origin
More Product Data
Luciferase assays in hard-to-transfect Jurkat cells
Related Products
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cloning MethodRestriction Enzyme/MCS
Delivery TypeTransfection
DescriptionpTK-Green Renilla Luc Vector
For Use With (Application)Reporter Assays
Product LinePierce
Quantity10 μg
Reporter GeneGreen Renilla Luciferase
Selection Agent (Eukaryotic)Puromycin
PromoterTK
TypeLuc Vector
Unit SizeEach
Contents & Storage
Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: -70 to -20°C.