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Thermo Scientific™
pCMV-Gaussia-Dura Luc Vector for Luciferase Assays
The Thermo Scientific pCMV-Gaussia-Dura Luc vector is a derivative of pMCS-Gaussia-Dura Luc. It contains a mutant form of the GaussiaRead more
| Catalog Number | Quantity |
|---|---|
| 16191 | 10 μg |
Catalog number 16191
Price (CNY)
1,232.00
Each
Quantity:
10 μg
Price (CNY)
1,232.00
Each
The Thermo Scientific pCMV-Gaussia-Dura Luc vector is a derivative of pMCS-Gaussia-Dura Luc. It contains a mutant form of the Gaussia luciferase gene (conferring better bioluminescent signal stability than the native luciferase) under the control of the strong Cytomegalovirus (CMV) promoter. This constitutive expression vector can be used as a normalization control to account for experimental variation in combination with other reporters.
Features of the pCMV-Gaussia-Dura Luc vector:
• Strong (CMV) constitutive promoter for co-transfection and normalization
• Naturally-secreting Gaussia-Dura luciferase gene, optimized for high expression and glow-stability in mammalian systems
• Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another
• Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
• Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
• High-copy pUC bacterial DNA replication origin
Gaussia-Dura luciferase (approx. 20kDa) is a secreted protein that enables measurement of the reporter activity in media (for real-time assays) and in cell lysates. The parent pMCS vector contains a multiple cloning site for cloning a promoter to study its regulatory potential.
These vectors are subject to a limited use label license.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cloning MethodRestriction Enzyme/MCS
Delivery TypeTransfection
DescriptionpCMV-Gaussia-Dura Luc Vector
For Use With (Application)Reporter Assays
Quantity10 μg
Reporter GeneGaussia Dura Luciferase
Selection Agent (Eukaryotic)Puromycin
PromoterCMV
TypeLuc Vector
Unit SizeEach
Contents & Storage
Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: -70 to -20°C.