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Invitrogen™
Terminal Deoxynucleotidyl Transferase (TdT) Buffer
Terminal Deoxynucleotidyl Transferase, Recombinant (rTdT) is a DNA polymerase that catalyzes the addition of deoxynucleotides to the 3fi hydroxyl terminusRead more
| Catalog Number | Quantity |
|---|---|
| 16314015 | 2 x 0.8 mL |
Catalog number 16314015
Price (CNY)
399.00
Each
Quantity:
2 x 0.8 mL
Price (CNY)
399.00
Each
Terminal Deoxynucleotidyl Transferase, Recombinant (rTdT) is a DNA polymerase that catalyzes the addition of deoxynucleotides to the 3fi hydroxyl terminus of DNA. A TdT Technical Bulletin is available.
Applications: Homopolymer tailing of vector and insert for cloning. Labeling oligonucleotides with biotin (1,2), 32P- or 35S-label (3), or in apoptosis (TUNEL) (4,5).
Source: Purified from a baculovirus clone of calf thymus TdT.
Performance and Quality Testing: Endonuclease, 3´ and 5´ exodeoxyribonuclease, and levels of incorporation tested.
Unit Definition: One unit incorporates 1 nmol dATP into acid-precipitable material in 1 h at 37°C, using d(pA)50 as a primer.
Hazard Warning: Toxic; potassium cacodylate contained in reaction buffer. Also contains cobalt chloride, a highly toxic chemical.
See MSDS.
Unit Reaction Conditions: 0.2 M potassium cacodylate (pH 7.2), 10 mM MgO4 C4 H6 , 1 mM 2-mercaptoethanol, 0.5 mg/ml BSA,
100 flM d(pA)50, 1 mM [3H]dATP, and enzyme in 0.15 ml for 1 h at 37°C.
Applications: Homopolymer tailing of vector and insert for cloning. Labeling oligonucleotides with biotin (1,2), 32P- or 35S-label (3), or in apoptosis (TUNEL) (4,5).
Source: Purified from a baculovirus clone of calf thymus TdT.
Performance and Quality Testing: Endonuclease, 3´ and 5´ exodeoxyribonuclease, and levels of incorporation tested.
Unit Definition: One unit incorporates 1 nmol dATP into acid-precipitable material in 1 h at 37°C, using d(pA)50 as a primer.
Hazard Warning: Toxic; potassium cacodylate contained in reaction buffer. Also contains cobalt chloride, a highly toxic chemical.
See MSDS.
Unit Reaction Conditions: 0.2 M potassium cacodylate (pH 7.2), 10 mM MgO4 C4 H6 , 1 mM 2-mercaptoethanol, 0.5 mg/ml BSA,
100 flM d(pA)50, 1 mM [3H]dATP, and enzyme in 0.15 ml for 1 h at 37°C.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Compatible Buffer5X Buffer, Reaction Buffer
Product TypeTdT
Quantity2 x 0.8 mL
Shipping ConditionApproved for shipment on Wet or Dry Ice
Unit SizeEach
Contents & Storage
Terminal Deoxynucleotidyl Transferase, Recombinant (rTdT) is supplied with vial of 5X buffer [500 mM potassium cacodylate (pH 7.2), 10 mM CoCl2 , 1 mM DTT]. Store at -20°C.
Citations & References (1)
Citations & References
Abstract
Different effects on human topoisomerase I by minor groove and intercalated deoxyguanosine adducts derived from two polycyclic aromatic hydrocarbon diol epoxides at or near a normal cleavage site.
Journal:J Biol Chem
PubMed ID:11832494
Topoisomerase I (top1) relieves supercoiling in DNA by forming transient covalent cleavage complexes. These cleavage complexes can accumulate in the presence of damaged DNA or anticancer drugs that either intercalate or lie in the minor groove. Recently we reported that covalent diol epoxide (DE) adducts of benzo[a]pyrene (BaP) at the