B-27™ Supplement (50X), serum free
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B-27™ Supplement (50X), serum free
Citations & References (7)
Gibco™

B-27™ Supplement (50X), serum free

The original Gibco B-27 Supplement, optimized for serum-free culture of neurons, supports the growth and viability of neurons and many different cell types.
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Catalog NumberQuantity
1750404410 mL
17504001100 mL
Catalog number 17504044
Price (CNY)
1,158.00
飞享价
Ends: 27-Dec-2025
1,879.00
Save 721.00 (38%)
Each
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Quantity:
10 mL
Price (CNY)
1,158.00
飞享价
Ends: 27-Dec-2025
1,879.00
Save 721.00 (38%)
Each
Add to cart

This original and genuine B-27 Supplement is a cGMP-manufactured, serum-free solution designed to ensure the consistent, reliable, and high-quality growth of embryonic, post-natal, and adult hippocampal neurons, as well as other CNS neurons. With a proven track record, B-27 Supplement supports both low- and high-density cultures, ensuring viability in serum-free media. Each batch undergoes rigorous performance testing to confirm its ability to successfully culture hippocampal neurons, providing you with the confidence and dependability you need for your research.

  • Dependable consistency—every lot is performance-tested for reliable results
  • High quality—cGMP manufacturing provides the highest standards of quality
  • Versatile usage—ideal for use with serum-free media such as Neurobasal Medium or Neurobasal-A Medium, without the need for an astrocyte feeder layer. Supports the growth and viability of neurons, as well as many other cell types differentiated from PSCs.
  • Proven performance—a proprietary formulation; the first and most cited neuronal cell culture supplement, trusted by researchers worldwide

Choose B-27 Supplement for the authentic solution that sets the standard for neuronal cell culture.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeNeural Cells
Concentration50 X
Culture TypeMammalian Cell Culture
Product LineB-27
Quantity10 mL
Serum LevelSerum-Free
Shelf Life12 Months
Shipping ConditionDry Ice
SourceSerum-Free
Tested ForEndotoxin, Performance, Sterility, Mycoplasma
ClassificationSerum-free
FormLiquid
Product TypeCell Culture Supplement
SterilitySterile
pH6 to 8
Unit SizeEach
Contents & Storage
Store in freezer (-5 to -20°C) and protect from light.

Frequently asked questions (FAQs)

Can B-27 supplement be filtered?

Yes, the protein content in this product is high enough so filtering through a low protein binding filter as a 50X or 1X in solution should not be a problem.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can Neurobasal Medium alone or Neurobasal Medium supplemented with B-27 supplement be frozen to extend product shelf life?

No, it is not recommended to freeze media due to the potential of precipitates forming upon thaw. Inorganic salts and amino acids in the formulation may come out of solution when exposed to temperature fluctuations. These precipitates will not go back into solution easily once formed.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What supplements should I use with Neurobasal or Neurobasal-A medium?

Neurobasal and Neurobasal-A media (for postnatal and adult neurons) allow for long-term maintenance of neuronal cells without the need for an astrocyte feeder layer. These media should be supplemented with either serum or a serum-free supplement, plus 0.5mM L-glutamine. B-27 supplement is a serum-free supplement that comes as a 50X concentrate in a 10ml volume. This is enough supplement for 500ml of media. Fetal, postnatal, and adult neural cultures can be grown in the appropriate Neurobasal medium supplemented with B-27 supplement .

We also have two other supplements. One is called G-5 and is for growth and expression of glial cells (normal and tumor) of astrocytic phenotype. This comes in a 1ml size, at a 100X concentration. The other supplement is called N-2 and is for growth and expression of post-mitotic neurons and tumor cells of neuronal phenotype. This comes in a 5ml size, at a 100X concentration.

For more information on Neurobasal media, search "Neurobasal" from our website home page.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Which media system should I use for primary culture of P0- or P1-derived mixed hippocampal cells?

We recommend using Neurobasal Plus Medium with B-27 Plus Supplement for long-term culture of mixed hippocampal cells. For short-term culture of a pure population of hippocampal neurons, we would recommend using Neurobasal-A Medium with B-27 Supplement.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you offer B-27 supplements with and without insulin?

Yes, we do offer B-27 Supplement, minus insulin (Cat. Nos. A1895601 and A1895602). All our other B-27 supplements do contain insulin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all B-27™ Supplement (50X), serum free FAQs

Citations & References (7)

Citations & References
Abstract
A comparison between different human hepatocyte models reveals profound differences in net glucose production, lipid composition and metabolism in vitro.
Authors:Bonanini F,Singh M,Yang H,Kurek D,Harms AC,Mardinoglu A,Hankemeier T
Journal:Experimental cell research
PubMed ID:38499143
In vitro generation of human pluripotent stem cell derived lung organoids.
Authors:Dye BR, Hill DR, Ferguson MA, Tsai YH, Nagy MS, Dyal R, Wells JM, Mayhew CN, Nattiv R, Klein OD, White ES, Deutsch GH, Spence JR
Journal:
PubMed ID:25803487
'Recent breakthroughs in 3-dimensional (3D) organoid cultures for many organ systems have led to new physiologically complex in vitro models to study human development and disease. Here, we report the step-wise differentiation of human pluripotent stem cells (hPSCs) (embryonic and induced) into lung organoids. By manipulating developmental signaling pathways hPSCs ... More
Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro.
Authors:Spence JR, Mayhew CN, Rankin SA, Kuhar MF, Vallance JE, Tolle K, Hoskins EE, Kalinichenko VV, Wells SI, Zorn AM, Shroyer NF, Wells JM
Journal:Nature
PubMed ID:21151107
Studies in embryonic development have guided successful efforts to direct the differentiation of human embryonic and induced pluripotent stem cells (PSCs) into specific organ cell types in vitro. For example, human PSCs have been differentiated into monolayer cultures of liver hepatocytes and pancreatic endocrine cells that have therapeutic efficacy in ... More
Axonal Transport Enables Neuron-to-Neuron Propagation of Human Coronavirus OC43.
Authors:Dubé M, Le Coupanec A, Wong AHM, Rini JM, Desforges M, Talbot PJ
Journal:J Virol
PubMed ID:29925652
Human coronaviruses (HCoVs) are recognized respiratory pathogens for which accumulating evidence indicates that in vulnerable patients the infection can cause more severe pathologies. HCoVs are not always confined to the upper respiratory tract and can invade the central nervous system (CNS) under still unclear circumstances. HCoV-induced neuropathologies in humans are ... More
Recapitulation of SARS-CoV-2 infection and cholangiocyte damage with human liver ductal organoids.
Authors:Zhao B, Ni C, Gao R, Wang Y, Yang L, Wei J, Lv T, Liang J, Zhang Q, Xu W, Xie Y, Wang X, Yuan Z, Liang J, Zhang R, Lin X
Journal:Protein Cell
PubMed ID:32303993
View all B-27™ Supplement (50X), serum free citations