CaptureSelect™ FcXL Affinity Matrix
CaptureSelect™ FcXL Affinity Matrix
Thermo Scientific™

CaptureSelect™ FcXL Affinity Matrix

Thermo Scientific™ CaptureSelect™ FcXL Affinity Matrix has been specifically designed for the purification of recombinant human IgG, Fc fusion proteins, and plasma derived IgG.
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Catalog NumberQuantity
19432805L5 L
1943280055 mL
19432801010 mL
19432805050 mL
1943280250250 mL
19432801L1 L
194328010L10 L
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Catalog number 19432805L
Price (CNY)/ 5 L
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Quantity:
5 L

CaptureSelect FcXL Affinity Matrix has been specifically designed for the purification of recombinant human IgG, Fc fusion proteins, and plasma derived IgG. It recognizes the CH3 domain of all human IgG subclasses (IgG1, 2, 3, and 4).

Features of this affinity matrix include:

  • Mild elution, making it suitable for Fc fusion proteins
  • Human specific, no binding to bovine antibodies, for example
  • Excellent scalability
  • Non-animal-derived

CaptureSelect FcXL Affinity Matrix purifies IgGs and Fc fusion proteins directly from complex source materials in a single step with high purity and yield.

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main Characteristics:

  • Matrix: agarose-based, aldehyde-activated
  • Average particle size: 65 ± 10 μm
  • Ligand: CaptureSelect FcXL affinity ligand
  • Ligand coupling method: aldehyde coupling via NH2 residues of the ligand
  • Binding capacity: 25–35 g IgG/L resin depending on flow rate, column height, and contact time
  • Elution conditions: 20 mM citric acid or acetic acid, pH 3–4, or 20 mM sodium acetate, 1.0 M MgCl2, 40% (v/v) propylene glycol, pH 5–6
  • Flow characteristics: 50–200 cm/h (up to 2 bar)
  • Formulation buffer: 20%(v/v) ethanol
Specifications
Binding Capacity25 to 35 g IgG/L
Flow Rate50 to 200 cm/hr. (Up to 2 bar)
MatrixAgarose-based, aldehyde-activated
Particle Size65 ±10 μm
FormAgarose Bead
Product LineCaptureSelect™
Quantity5 L
For Use With (Application)IgG purification
TargetFc of IgG, CH3 domain
TypeAffinity Matrix
Ligand TypeFcXL affinity ligand
Stationary PhaseAnti-IgG-Fc
Column TypeBulk Resin
Unit Size5 L
Contents & Storage
Store in refrigerator (2°C to 8°C).