PBS, pH 7.2

Gibco Phosphate-Buffered Saline (PBS), pH 7.2, is a balanced salt solution widely used in various cell culture applications. It is designed to maintain a stable pH and osmotic balance, making it ideal for washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.

Key features

• Without calcium and magnesium—the absence of calcium and magnesium is crucial for applications requiring the rinsing of chelators from the culture before cell dissociation
• Without phenol red—the lack of phenol red ensures that there is no interference with optical measurements and prevents potential effects on cell physiology

Applications

• Cell washing—ideal for washing cells before dissociation to remove any residual enzymes or chelators
• Sample transport—suitable for transporting cells or tissue samples while maintaining their viability
• Cell counting—can be used to dilute cells for accurate counting
• Reagent preparation—provides a stable environment for preparing various reagents used in cell culture

Why choose PBS vs. DPBS

• Simplicity—PBS is a simpler formulation compared to Dulbecco's Phosphate-Buffered Saline (DPBS), which includes additional components like potassium chloride and may be formulated with or without calcium, magnesium, glucose, and pyruvate. This simplicity makes PBS a versatile choice for general cell culture applications.
• Specific applications—PBS is preferred for applications where the presence of potassium or other additives in DPBS might interfere with experimental outcomes

Why choose pH 7.2 vs. pH 7.4 PBS

• Experimental requirements—the choice between pH 7.2 and pH 7.4 depends on the specific requirements of your cell culture system. pH 7.2 is often used for certain cell types and experimental conditions where a slightly more acidic environment is beneficial.
• Buffering capacity—pH 7.2 provides a slightly different buffering capacity, which can be crucial for maintaining the desired physiological conditions in specific experimental setups

Why choose Gibco PBS, pH 7.2

• Quality and consistency—Gibco products are manufactured in cGMP, ISO-certified facilities, ensuring the highest quality and consistency. Each batch undergoes rigorous testing for osmolality, pH levels, sterility, and the presence of endotoxins.
• Track record—Gibco cell culture products have a long-standing reputation for excellence in the field of cell culture, with numerous publications citing the use of Gibco products. This track record of reliability and performance makes Gibco PBS the preferred choice for researchers worldwide.

Gibco PBS, pH 7.2 is a reliable and consistent solution for a wide range of applications. Choose Gibco products for your cell culture needs and benefit from our decades of expertise and innovation in the field.