MEM, glutamine, HEPES, powder
MEM, glutamine, HEPES, powder
Gibco™

MEM, glutamine, HEPES, powder

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be usedRead more
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Catalog NumberQuantity
2101104450 L
Catalog number 21011044
Price (CNY)
-
Quantity:
50 L
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Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco™ MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM is modified as follows:

With Without
• L-glutamine • Sodium Bicarbonate
• Phenol Red
• HEPES

The complete formulation is available.

Gibco™ MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. MEM is available with Earle’s salts for use in a CO2 incubator, or with Hanks' salts for use without CO2. This product is made with Earle’s salts.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

cGMP Manufacturing and Quality System
Gibco™ MEM is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Powder forms of Gibco™ L-15 cell culture medium require pH adjustment and filtration at the time of preparation (see protocol for details).
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeMammalian Cells
Culture EnvironmentCO2
Osmolality240 - 280 mOsm/kg
Product LineGibco™
Product TypeMinimum Essential Medium (MEM)
Quantity50 L
Shelf Life36 Months
Shipping ConditionRoom Temperature
ClassificationAnimal Origin-free
Culture TypeMammalian Cell Culture
FormPowder
Serum LevelStandard Serum Supplementation
With AdditivesGlutamine, HEPES, Phenol Red
Without AdditivesNo Sodium Bicarbonate, No Sodium Pyruvate
pH5.2 to 5.6
Unit SizeEach
Contents & Storage
Storage conditions: 2-8° C.
Shipping conditions: Ambient.
Shelf life: 36 months from date of manufacture.

Frequently asked questions (FAQs)

Where can I find the osmolality for MEM Medium?

The osmolality is listed in the COA for the particular lot number of the medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the shelf life of my powdered media once I reconstitute it?

Expiration date of most Gibco reconstituted dry format media (AGT or DPM) has not been established; end users should assess performance and stability of this reconstituted media in a system that is relevant to their process. A dry format product (either DPM or AGT) will age upon storage and while we may not be able to detect which component(s) degrade since we can't measure every component, that doesn't mean that the reconstituted liquid will have the same stability as a fresh liquid made by direct weigh methods.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.