MBS (m-maleimidobenzoyl-N-hydroxysuccinimide ester)
MBS (m-maleimidobenzoyl-N-hydroxysuccinimide ester)
Citations & References (3)
Thermo Scientific™

MBS (m-maleimidobenzoyl-N-hydroxysuccinimide ester)

Thermo Scientific Pierce MBS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of aRead more
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Catalog NumberQuantity
2231150 mg
Catalog number 22311
Price (CNY)
1,827.00
Each
Add to cart
Quantity:
50 mg
Price (CNY)
1,827.00
Each
Add to cart
Thermo Scientific Pierce MBS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a short aromatic spacer arm (7.3 angstroms).

Features of MBS:

• Reactive groups: NHS ester and maleimide
• Reactive towards: amino and sulfhydryl groups
• Non-cleavable
• Water-insoluble (dissolve first in DMF or DMSO); compare to Sulfo-MBS
• Popular for forming enzyme immunoconjugates

Product References:
Crosslinker Application Guide -- search for recent literature references for this product

Related Products
Sulfo-MBS (m-maleimidobenzoyl-N-hydroxysulfosuccinimide ester)
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell PermeabilityYes
DescriptionMBS
FormPowder
Labeling MethodChemical Labeling
Molecular Weight (g/mol)314.25
PEGylatedNo
Product LinePierce
Quantity50 mg
Reactive MoietyMaleimide, NHS Ester
Shipping ConditionAmbient
SolubilityDMF, DMSO
Spacer Arm Length7.3 Å
Water SolubleNo
Chemical ReactivityAmine-Sulfhydryl
CleavableNo
Crosslinker TypeHeterobifunctional
FormatStandard
Product TypeCrosslinker
SpacerShort (<10 Å)
Unit SizeEach
Contents & Storage
Upon receipt store desiccated at 4°C.

Frequently asked questions (FAQs)

Can you provide the shelf-life for MBS (m-maleimidobenzoyl-N-hydroxysuccinimide ester)?

MBS (m-maleimidobenzoyl-N-hydroxysuccinimide ester) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Citations & References (3)

Citations & References
Abstract
Solid phase DNA amplification: characterisation of primer attachment and amplification mechanisms.
Authors:Adessi C, Matton G, Ayala G, Turcatti G, Mermod JJ, Mayer P, Kawashima E
Journal:Nucleic Acids Res
PubMed ID:11024189
Different chemical methods used to attach oligonucleotides by their 5'-end on a glass surface were tested in the framework of solid phase PCR where surface-bound instead of freely-diffusing primers are used to amplify DNA. Each method was first evaluated for its capacity to provide a high surface coverage of oligonucleotides ... More
Covalent attachment of synthetic DNA to self-assembled monolayer films.
Authors:Chrisey LA, Lee GU, O'Ferrall CE
Journal:Nucleic Acids Res
PubMed ID:8760890
'The covalent attachment of thiol-modified DNA oligomers; to self-assembled monolayer silane films on fused silica and oxidized silicon substrates is described. A heterobifunctional crosslinking molecule bearing both thiol- and amino-reactive moieties was used to tether a DNA oligomer (modified at its terminus with a thiol group) to an aminosilane film ... More
Spatial and dynamic interactions between phospholamban and the canine cardiac Ca2+ pump revealed with use of heterobifunctional cross-linking agents.
Authors:Chen Z, Stokes DL, Rice WJ, Jones LR
Journal:J Biol Chem
PubMed ID:12972413
Heterobifunctional thiol to amine cross-linking agents were used to gain new insights on the dynamics and conformational factors governing the interaction between the cardiac Ca2+ pump (SERCA2a) and phospholamban (PLB). PLB is a small protein inhibitor of SERCA2a that reduces enzyme affinity for Ca2+ and thereby regulates cardiac contractility. We ... More