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Gibco™
MEM α, nucleosides
MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negativeRead more
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| Catalog Number | Quantity |
|---|---|
| 22571038 also known as 22571-038 | 10 x 500 mL |
| 12571063 | 500 mL |
| 12571071 | 10 x 500 mL |
| 12571048 | 1000 mL |
| 12571089 | 6 x 1000 mL |
Catalog number 22571038
also known as 22571-038
Price (CNY)
-
Quantity:
10 x 500 mL
MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of MEM α modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This MEM α is modified as follows:
| With |
| • Ribonucleosides |
| • Deoxyribonucleosides |
| • Phenol Red |
| • L-glutamine |
The complete formulation is available.
Using MEM α
MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR-negative cells. This product is made with Earle’s salts. MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Cell TypeMammalian Cells
Concentration1 X
Culture EnvironmentCO2
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Osmolality280 - 310 mOsm/kg
Product LineGibco
Product TypeMEM α (Minimum Essential Medium α)
Quantity10 x 500 mL
Shelf Life12 Months
ClassificationAnimal Origin-free
Culture TypeMammalian Cell Culture
FormLiquid
Serum LevelStandard Serum Supplementation
SterilitySterile-filtered
Sterilization MethodSterile-filtered
With AdditivesGlutamine, Phenol Red, Sodium Pyruvate, Ribonucleosides, Deoxyribonucleosides, Sodium Bicarbonate
Without AdditivesNo HEPES
pH7 to 7.4
Unit SizeEach
Contents & Storage
Storage conditions: 2-8° C. Protect from light.
Shipping conditions: Ambient.
Shelf life: 12 months from date of manufacture.
Shipping conditions: Ambient.
Shelf life: 12 months from date of manufacture.