SuperSignal™ West Pico Rabbit IgG Detection Kit

Thermo Scientific SuperSignal West Pico Complete Rabbit IgG Detection Kit is a a kit containing goat anti-rabbit secondary antibody HRPRead more

Catalog Number	Quantity
34083	500 mL

Catalog number 34083

Price (CNY)

6,164.00

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Quantity:

500 mL

Request bulk or custom format

Price (CNY)

6,164.00

Add to cart

Thermo Scientific SuperSignal West Pico Complete Rabbit IgG Detection Kit is a a kit containing goat anti-rabbit secondary antibody HRP conjugate for the detection of rabbit primary antibody. The kit is ideal for busy researchers or new beginners to Western blot analysis.

Features of SuperSignal West Pico Complete Rabbit IgG Detection Kit:

• Optimized—secondary antibodies are validated for use with the SuperSignal West Pico Chemiluminescent Substrate, eliminating the need to test multiple dilutions
• Large packages—kits provide sufficient reagents to complete up to 50 experiments with standard mini gel blots (4000 square centimeters of membrane)
• Standard or complete—choose a kit with substrate and antibody only, or a complete kit that also includes TBS wash buffer and high-performance SuperBlock Blocking Buffer

All the components of these Western blotting kits are optimized for compatibility the SuperSignal West Pico Chemiluminescent Substrate. The kits are offered in a standard format (substrate and optimized HRP-conjugates) and a complete format that also includes the Thermo Scientific SuperBlock Buffer and BupH Tris Buffered Saline.

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SuperSignal™ West Pico Mouse IgG Detection Kit
SuperSignal™ West Pico Complete Rabbit IgG Detection Kit

For Research Use Only. Not for use in diagnostic procedures.

Specifications

ConjugateHRP

DescriptionSuperSignal West Pico Rabbit IgG Detection Kit

ReactivityRabbit

Sufficient For50 mini blots (4000 sq. cm of membrane)

Detection MethodChemiluminescence

For Use With (Application)Western Blot

Membrane CompatibilityNitrocellulose, PVDF

Product LineSuperSignal

Product TypeRabbit IgG Detection Kit

Quantity500 mL

SubstrateHorseradish Peroxidase

Unit SizeEach

Contents & Storage

Sufficient For: ∼62 mini blots probed with rabbit primary antibodies
• SuperSignal West Pico Luminol/Enhancer Solution, 250 mL
• SuperSignal West Pico Stable Peroxide Solution, 250 mL
• Peroxidase Conjugated Goat anti-Rabbit IgG (H + L), 2 mL

Frequently asked questions (FAQs)

How can I increase the sensitivity of my Western blot?

Consider transferring to a different membrane or using a different detection method. We have observed increased sensitivity when using PVDF membranes in place of nitrocellulose. On PVDF membranes, using as little as 1 µg of total rat brain protein, PKC can be detected with alkaline phosphatase-mediated chromogenic detection in some cases using affinity-purified antibodies at a concentration of 0.5 µg/mL. Detection sensitivity can also be increased by using chemiluminescent detection, especially when using a SuperSignal West enhanced chemiluminescence subtrate (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/western-blot-detection-reagents/detection-technologies-western-blotting/chemiluminescent-western-blot-detection/supersignal-chemiluminescent-substrates.html) such as SuperSignal West Pico PLUS, SuperSignal West Dura, or SuperSignal West Femto. The secondary antibody should be used as recommend by the manufacturer and optimized as needed.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.