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Thermo Scientific™
Rapid ELISA Mouse mAb Isotyping Kit
The Thermo Scientific Pierce Rapid ELISA Mouse mAb Isotyping Kit provides antigen-independent determination of mouse monoclonal antibody isotype using aRead more
| Catalog Number | Quantity |
|---|---|
| 37503 | 60 Tests |
Catalog number 37503
Price (CNY)
8,392.00
Each
Quantity:
60 Tests
Price (CNY)
8,392.00
Each
The Thermo Scientific Pierce Rapid ELISA Mouse mAb Isotyping Kit provides antigen-independent determination of mouse monoclonal antibody isotype using a microplate method that enables multiple samples to be tested in about one hour.
Features of the Rapid ELISA Mouse mAb Isotyping Kit:
• Fast—determine antibody isotype in approx. 1 hour; considerably faster than traditional ELISA kits
• Convenient—eight-well strip format allows partial use of the plate; use one strip (column) for each sample (12 samples per plate)
• Specific—characterize antibodies for six different subclasses and two different light-chain types
• Sensitive—accurate characterization with samples containing at least 3 ng/mL of test antibody
• Flexible—kit is compatible with hybridoma cell culture supernatant, ascites fluid or purified antibodies
• Cost effective—cost per assay is less than with single-use assay strips or cassettes
• No special equipment needed—assess results visually or measure quantitatively using an ordinary ELISA plate reader (450nm)
• Complete and easy to use—includes precoated plates, detecting antibody, buffers, and TMB substrate and stop solutions
This fast assay uses ELISA strip-plates that are pre-coated in different wells with anti-mouse heavy-chain capture antibodies (anti-IgG1, IgG2a, IgG2b, IgG3, IgA and IgM) or anti-mouse light-chain capture antibody (kappa or lambda). This approach eliminates the need to purify and immobilize an antigen to determine immunoglobulin isotype (antibody subclass and light chain identity). A mouse monoclonal antibody sample applied to the wells can be isotyped within one hour. Results are evaluated qualitatively by visual inspection or quantitatively by measuring the absorbance at 450nm.
Related Products
Pierce™ Rapid Antibody Isotyping Kit - Mouse
Pierce™ Rapid Antibody Isotyping Kit plus Kappa and Lambda - Mouse
Features of the Rapid ELISA Mouse mAb Isotyping Kit:
• Fast—determine antibody isotype in approx. 1 hour; considerably faster than traditional ELISA kits
• Convenient—eight-well strip format allows partial use of the plate; use one strip (column) for each sample (12 samples per plate)
• Specific—characterize antibodies for six different subclasses and two different light-chain types
• Sensitive—accurate characterization with samples containing at least 3 ng/mL of test antibody
• Flexible—kit is compatible with hybridoma cell culture supernatant, ascites fluid or purified antibodies
• Cost effective—cost per assay is less than with single-use assay strips or cassettes
• No special equipment needed—assess results visually or measure quantitatively using an ordinary ELISA plate reader (450nm)
• Complete and easy to use—includes precoated plates, detecting antibody, buffers, and TMB substrate and stop solutions
This fast assay uses ELISA strip-plates that are pre-coated in different wells with anti-mouse heavy-chain capture antibodies (anti-IgG1, IgG2a, IgG2b, IgG3, IgA and IgM) or anti-mouse light-chain capture antibody (kappa or lambda). This approach eliminates the need to purify and immobilize an antigen to determine immunoglobulin isotype (antibody subclass and light chain identity). A mouse monoclonal antibody sample applied to the wells can be isotyped within one hour. Results are evaluated qualitatively by visual inspection or quantitatively by measuring the absorbance at 450nm.
Related Products
Pierce™ Rapid Antibody Isotyping Kit - Mouse
Pierce™ Rapid Antibody Isotyping Kit plus Kappa and Lambda - Mouse
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)ELISA (Antigen Capture), Absorbance
For Use With (Equipment)Microplate Reader
Quantity60 Tests
AssayIsotyping Assay
Detection MethodColorimetric
Product LinePierce
Target SpecificityTarget-specific
Unit SizeEach
Contents & Storage
Sufficient For: 60 isotype determinations (8 wells per test sample)
• Pre-coated 96-well Isotyping Plates, 5 plates
• Goat Anti-Mouse IgG+IgA+IgM-HRP Conjugate, 30 mL
• TMB Substrate, 55 mL
• Stop Solution, 55 mL
• 30X Wash Buffer, 200 mL
• TBS Buffer Packs (each makes 500 mL), 2 packs
Store at 4°C.
• Pre-coated 96-well Isotyping Plates, 5 plates
• Goat Anti-Mouse IgG+IgA+IgM-HRP Conjugate, 30 mL
• TMB Substrate, 55 mL
• Stop Solution, 55 mL
• 30X Wash Buffer, 200 mL
• TBS Buffer Packs (each makes 500 mL), 2 packs
Store at 4°C.