SuperBlock™ Blocking Buffer
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SuperBlock™ Blocking Buffer
Thermo Scientific™

SuperBlock™ Blocking Buffer

SuperBlock Blocking Buffer is an optimized solution containing a single purified glycoprotein that provides incredibly fast and effective blocking for ELISA, IHC, and western blot analysis.
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Catalog NumberQuantityChemical Name or Material
375455 packsSuperBlock (TBS) Dry Blend
37580100 mLSuperBlock (PBS)
375151 LSuperBlock (PBS)
375185 LSuperBlock (PBS)
37581100 mLSuperBlock (TBS)
375351 LSuperBlock (TBS)
375161 LSuperBlock T20 (PBS)
375361 LSuperBlock T20 (TBS)
Catalog number 37545
Price (CNY)
3,133.00
Each
Add to cart
Quantity:
5 packs
Chemical Name or Material:
SuperBlock (TBS) Dry Blend
Request bulk or custom format
Price (CNY)
3,133.00
Each
Add to cart
SuperBlock Blocking Buffer is an optimized solution containing a single purified glycoprotein that provides incredibly fast and effective blocking for ELISA, IHC, and western blot analysis. Blocking micro-plates, membranes, or tissues with a SuperBlock buffer yields a high signal-to-noise ratio in most detection systems.
Features of SuperBlock Blocking Buffer include:
Blocking time—block membranes in 5 to 10 minutes and ELISA plates in 2 minutes
Applications—ELISA, western blotting, IHC; biotin-free for use with streptavidin system
Stability—store buffer at 4°C for one year; store blocked plates dry for up to 12 months

Blocking micro-plates, membranes, or tissues with SuperBlock buffer yields a high signal-to-noise ratio in most detection systems. The protein-based formulation does not contain any immunoglobulins, albumin, or endogenous biotin, making it compatible in many situations where traditional blocking agents fail. The buffer is especially effective at blocking coated polystyrene microplates (96-well plates) and stabilizing them for drying and storage for later use.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Chemical Name or MaterialSuperBlock (TBS) Dry Blend
Recommended StorageUpon receipt store at 4°C. Product is shipped at ambient temperature.
For Use With (Application)ELISA
Physical FormPowder
Product LineSuperBlock™
Quantity5 packs
Unit SizeEach

Frequently asked questions (FAQs)

What incubation time and temperature should I use for effective blocking using the SuperBlock Blocking Buffer ?

Typical procedures use 1 hour at room temperature or overnight at 4°C. However, sufficient blocking in many procedures is possible in 3 x 2 minutes at room temperature.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Is SuperBlock Blocking Buffer RNase-free?

No, SuperBlock Blocking Buffer is not RNase-free.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Does SuperBlock Blocking Buffer work with nylon and nitrocellulose membrane?

Yes, SuperBlock Blocking Buffer works with nylon and nitrocellulose membrane.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Does SuperBlock Blocking Buffer need to be diluted?

No. It is supplied at 1X concentration and should be used “as is.“

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Does SuperBlock Blocking Buffer contain albumin, biotin, DNA, detergent or phosphoproteins?

No. Unless it includes “T20“ in the name, for example, SuperBlock T20 (PBS) Blocking Buffer, which contains 0.05% Tween 20.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.