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Thermo Scientific™
PNPP Substrates
Thermo Scientific™ Pierce PNPP Substrates are powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications.
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| Catalog Number | Format |
|---|---|
| 37620 | Kit |
| 34045 | Powder |
| 34047 | Tablet |
| 37621 | Liqiuid |
Catalog number 37620
Price (CNY)
4,512.00
Each
Format:
Kit
Price (CNY)
4,512.00
Each
PNPP (p-Nitrophenyl Phosphate, Disodium Sal) is a widely used substrate for detecting alkaline phosphatase in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405 nm. Pierce PNPP is available in four formats: dry crystalline powder, single-use tablets, a stable substrate solution, and a complete kit containing substrate solution and diethanolamine buffer.
Thermo Scientific Pierce PNPP (p-Nitrophenyl Phosphate, Disodium Salt) is a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405 nm.
Features of PNPP (p-nitrophenyl phosphate):
• One-component alkaline phosphatase (AP) substrate
• Absorbance maximum of 405 nm
• Develops a yellow hue in solution upon reaction with AP
• Safer to use—contains a non-mercury, non-azide preservative
The substrate is offered in several formats:
PNPP Substrate
• Powder Form
PNPP Substrate Tablets
• 5 mg tablets with an inert filler as the balance
PNPP Substrate Kit
• PNPP tablets and a Diethanolamine Buffer to yield more than one liter of substrate. The Diethanolamine Substrate Buffer (Cat. No. 34064) is also provided individually as a 5X concentrate.
1-Step™ PNPP Substrate Solution
• Circumvents this time-consuming and variable introducing step by providing a single component PNPP substrate. This substrate is stable for 12 months at 2–8°C and can be stopped with conventional methods.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionPNPP Substrate Kit
Detection MethodColorimetric
FormatKit
Product LinePierce™
Substrate PropertiesChemical Substrate
Substrate TypeAlkaline Phosphatase
Product TypeELISA Substrate
Quantity105 tablets kit
Unit SizeEach
Contents & Storage
Sufficient for: 5 mL of typical 1X substrate solution per tablet (525 mL total, approx. 5250 wells at 100 μL per well)
• Diethanolamine Buffer, 225 mL
• PNPP Tablets, 105 tablets (5 mg/tablet)
Store buffer at 4°C and PNPP tablets at -20°C.
• Diethanolamine Buffer, 225 mL
• PNPP Tablets, 105 tablets (5 mg/tablet)
Store buffer at 4°C and PNPP tablets at -20°C.