MEM, NEAA, powder
MEM, NEAA, powder
Gibco™

MEM, NEAA, powder

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be usedRead more
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Catalog NumberQuantity
4150008350 L
4150003410 x 1 L
4150001810 L
Catalog number 41500083
Price (CNY)
2,354.00
Each
Add to cart
Quantity:
50 L
Customize this product
Price (CNY)
2,354.00
Each
Add to cart

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco™ MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM is modified as follows:

With: NEAA, L-glutamine, Phenol Red

Without: HEPES, Sodium Bicarbonate

The complete formulation is available.

Gibco™ MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow's MEM, MEM α, DMEM, and Temin's Modification. MEM is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2. This product is made with Earle's salts.

Product Intended Use

For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH. Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

Please note that the shelf life on delivered products varies. This product has a minimum shelf life of 6 months at time of shipment. For more information regarding product shelf life, including details on specific product lots, please contact Customer Service.

Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
Specifications
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™
Quantity50 L
Shipping ConditionRoom Temperature
FormPowder
Serum LevelStandard Serum Supplementation
With AdditivesLow Glucose, Glutamine, Phenol Red
Without AdditivesNo HEPES, No Sodium Pyruvate
Unit SizeEach
Contents & Storage
Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture

Frequently asked questions (FAQs)

Do you offer an alternative for the discontinued EMEM (Cat. No. 670086)?

Unfortunately, we do not have a direct replacement for EMEM (Cat. No. 670086). The best alternative is to use powder MEM with NEAA (Cat. No. 41500018) and then add bicarbonate to 1.5 g/L and pyruvate to 1 mM final. This will almost be an exact replacement, but relies on having to make and filter a liquid from the powder.

The next best alternative is to use liquid MEM with NEAA (Cat. No. 10370021). This would require the addition of glutamine (2 mM final) and pyruvate (1 mM final) to equal the concentrations in the discontinued product. In this version, the bicarbonate concentration would be higher (2.2 g/L) than that in the discontinued product (1.5 g/L).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Where can I find the osmolality for MEM Medium?

The osmolality is listed in the COA for the particular lot number of the medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the shelf life of my powdered media once I reconstitute it?

Expiration date of most Gibco reconstituted dry format media (AGT or DPM) has not been established; end users should assess performance and stability of this reconstituted media in a system that is relevant to their process. A dry format product (either DPM or AGT) will age upon storage and while we may not be able to detect which component(s) degrade since we can't measure every component, that doesn't mean that the reconstituted liquid will have the same stability as a fresh liquid made by direct weigh methods.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

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