TaqMan™ MGB PROBE
Product Image
Thermo Scientific™

TaqMan™ MGB PROBE

Green features
Custom TaqMan™ MGB™ Probes are dual-labeled probes that can be used in all of your real-time and end-point PCR applications.•Read more
Have Questions?
Change viewbuttonViewtableView
Catalog NumberQuantity
43160340110 nmol
43160330120 nmol
43160320150 nmol
Catalog number 431603401
Price (CNY)
2,551.00
Each
Order now
Quantity:
10 nmol
Custom TaqMan™ MGB™ Probes are dual-labeled probes that can be used in all of your real-time and end-point PCR applications.

• Choice of reporter dyes, and synthesis scales
• Probes in Liquid with fixed concentration, easy to use
• Available for any species or organism
• For use in quantitative gene expression, SNP genotyping, other allelic discrimination applications, and pathogen detection

Applied Biosystems Custom TaqMan™ MGB™ Probes incorporate a 5’ reporter dye and a 3’ non-fluorescent quencher (NFQ). The NFQ offers the advantage of lower background signal, which results in better precision in quantitation. The MGB moiety stabilizes the hybridized probe and effectively raises the melting temperature (Tm). This means MGB™ probes can be shorter than traditional dual-labeled probes, which makes them better suited for allelic discrimination applications.

China-made Custom TaqMan MGB™ probes are available with the following reporter dyes; FAM™, VIC™, and NED™. All Custom TaqMan™ MGB™ Probes are HPLC purified. Design Custom TaqMan™ MGB™ Probes for any species, splice variant, or novel gene.

Convenient Ordering and Delivery
Now some Custom TaqMan™ MGB™ Probes are manufactured in China, with the same quality as those made in US, while the turnaround time shorten to 5-7 working days. TaqMan™ MGB™ Probes have a shelf life of 12 months from manufacturing date.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Contact Us
Please download the electronic order file, and send it to synsh@invitrogen.com.

Hotline: 800-820-8982 / 400-820-8982
Specifications
Green FeaturesSustainable packaging
Product LineTaqMan™
Product TypeTaqMan Probe
Purity or Quality GradeHPLC
Quantity10 nmol
Shipping ConditionApproved for shipment on Wet or Dry Ice
For Use With (Application)Real Time PCR (qPCR)
FormLiquid
Unit SizeEach
Contents & Storage
-20°C

Frequently asked questions (FAQs)

What are the key features of a TaqMan MGB probe?

TaqMan MGB probes have a nonfluorescent quencher at the 3´ end and a minor groove binder at the 3´ end that increases the melting temperature (Tm) of probe, allowing the use of shorter probes.  For more information on MGB probes, please consult the following manual: "Primer Express v1.5 and TaqMan MGB Probes for Allelic Discrimination: All PCR Instruments: User Bulletin." You can find a copy on our website by entering this title in the main search box.

When using the TaqMan Gene Signature Plates, should I mix the reactions after adding the master mix and template to the wells containing lyophilized primers and probe?

No, but it is recommended that the plate be centrifuged to draw down the liquid to the bottom of the wells.

What are your recommendations for the best way to dilute and store my TaqMan probe?

The probe should be diluted in TE buffer [10 mM Tris-HCl, pH 8.0 (at 25°C), 1 mM EDTA]. We recommend storing your probes, both stocks and working solutions, at -20°C in aliquots. This is to cut down on the number of times the probe is subject to freeze-thaws. For detailed information on diluting primers and probes, please refer to the tutorial entitled "Reconstituting and Diluting Primers and TaqMan Probes". You can find it on our website by entering this title in the main search field.

Find additional tips, troubleshooting help, and resources within ourTaqMan Primers and Probes Support Center.