TaqMan™ RNase P Detection Reagents Kit
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Applied Biosystems™

TaqMan™ RNase P Detection Reagents Kit

The Applied Biosystems™ TaqMan™ RNase P Detection Reagents kit provides the components needed to detect and quantitate genomic copies ofRead more
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Catalog NumberQuantity
4316831100 reactions
Catalog number 4316831
Price (CNY)
3,533.00
Each
Add to cart
Quantity:
100 reactions
Price (CNY)
3,533.00
Each
Add to cart
The Applied Biosystems™ TaqMan™ RNase P Detection Reagents kit provides the components needed to detect and quantitate genomic copies of the human RNase P gene using the 5' nuclease assay. Human RNase P gene is a single-copy gene encoding the RNA moiety for the RNase P enzyme. This 5' nuclease assay employs TaqMan™ Universal PCR Master Mix or TaqMan™ PCR Core Reagents for polymerase chain reaction (PCR) using the provided human genomic DNA as template.

• Pre-designed primers and TaqMan™ probe eliminate assay design
• Easy-to-use primer and TaqMan™ probe mix minimizes assay set-up time
• Rapid Assay Development Guidelines can minimize optimization time

Comprehensive Guidelines Included
Applied Biosystems™ has developed a comprehensive set of guidelines to ensure success when using Applied Biosystems™ Real-Time PCR reagents and instrumentation. These guidelines are simple and easy to follow. Many traditional variables, such as magnesium chloride concentration and the thermal cycling protocol itself, have been standardized, greatly reducing assay development time.

Normalization Built-In
All Applied Biosystems™ Real-Time PCR reagent kits contain a passive internal reference to normalize non-PCR related fluorescence fluctuations. Normalizing with a passive internal reference minimizes well-to-well variability that can result from a variety of causes, such as pipetting errors and sample evaporation. The passive reference is also essential for accurate results when multiple probes (with different reporter dyes) are combined in a single tube.

Note: See user's manual or package insert for limited label license, and trademark information. For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Calibration TypePassive Internal Reference Standards
Control TemplateHuman Genomic DNA
FormatTube
Internal Probe ModificationTAMRA™ Quencher (3'), FAM (5')
No. of Reactions100 Reactions
Product LineTaqMan
Product TypeReal Time PCR Reagents
Quantity100 reactions
Shipping ConditionDry Ice
SpeciesHuman
Sufficient For100 Reactions
TargetRNase P
GC-Rich PCR PerformanceLow
Label or DyeFAM
PCR MethodqPCR
Reaction SpeedStandard
Unit SizeEach
Contents & Storage
Control Primer-Probe Set(s) & Template(s)

Frequently asked questions (FAQs)

With the Oncomine Focus Assay, Chef-Ready Library kit, where can I find the combined barcoded AmpliSeq libraries when the run is complete?

After the completion of a run, the Ion Chef Instrument holds the barcoded libraries in the tube loaded in Position D of the Reagents cartridge. To avoid fluid loss due to evaporation, remove and cap the tube of combined barcoded libraries as soon as possible after run completion. Do not leave the tube in the instrument longer than 24 hours after the start of the run. After 24 hours from the start of the run, the instrument chiller will stop actively cooling, and the sample will be held at 27 degrees C.

The tube in Position D of the Ion AmpliSeq Chef Reagents DL8 Cartridge will contain 700 µL of combined barcoded libraries at completion of the run. The libraries are at >>100 pM (total combined library concentration) and are ready to use in template preparation. One can store unused portions of combined libraries at 4 to 8 degrees C for up to 1 month. For longer-term storage, store at -30 to -10 degrees C.

With the Oncomine Focus Assay, Chef-Ready Library kit, can I process fewer than 8 samples per Ion AmpliSeq run on the Ion Chef System?

Yes, fewer than 8 samples may be processed per run but keep in mind that a run consumes kit reagents for 8 samples regardless of the sample number.

If I process less than 8 samples using the Oncomine Focus Assay, Chef-Ready Library kit, do I need to quantitate the final combined output library?

If you are processing ≤5 samples, we recommend that you quantify your output combined library by qPCR to ensure that an optimal concentration is used in templating reactions.

If you are processing fewer than 8 samples, it is preferable to add replicates or positive control samples to the run. Otherwise, pipet 15 µL of nuclease-free water as non-template control into column 1 wells that do not contain a DNA sample.

What is the RNA input for the Oncomine Focus Assay, Chef-Ready Library kit?

Each reverse transcription reaction requires 10 ng of DNase-treated total RNA (≥1.43 ng/µL).

What is the DNA input for the Oncomine Focus Assay, Chef-Ready Library kit?

For each target amplification reaction, use 300-30,000 copies of DNA (10 ng of mammalian gDNA) from normal or FFPE tissue.