MicroSEQ™ Full Gene 16S rDNA Sequencing Kit
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Applied Biosystems™

MicroSEQ™ Full Gene 16S rDNA Sequencing Kit

The MicroSEQ™ Full Gene 16S rDNA Sequencing Kit is the sequencing component of the MicroSEQ™ 16S rDNA Full Gene IdentificationRead more
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Catalog NumberQuantity
43474841 Kit
Catalog number 4347484
Price (CNY)
12,306.00
Each
Add to cart
Quantity:
1 Kit
Price (CNY)
12,306.00
Each
Add to cart

The MicroSEQ™ Full Gene 16S rDNA Sequencing Kit is the sequencing component of the MicroSEQ™ 16S rDNA Full Gene Identification System, which provides an easy-to-use DNA sequence-based method to identify most bacteria. Use this kit to sequence PCR products that have been generated using the MicroSEQ™ Full Gene 16S rDNA PCR Kit. The two kits together permit the quick and convenient amplification and sequencing of the bacterial 16S rRNA gene. This kit includes a Protocol and a Quick Reference Card. The sequencing kit does not include a protocol or a Quick Reference Card.

  • Sequence-based measurement ensures you get reproducible results in under 24 hours.
  • Growth requirement-independent measurement enables you to identify bacteria using objective criteria.
  • Universal domain-level primers eliminate up-front optimization.
  • MicroSEQ™ ID Analysis Software ensures accurate species identification.
  • Customizable libraries give you the flexibility to create new libraries and search validated libraries that contain the most frequently encountered organisms.
  • Requires minimal training-similar work flow as the MicroSEQ™ D2 LSU fungal kit and the MicroSeq™ 500 16S rDNA kit.

The MicroSEQ™ ID System: A Complete, Integrated Solution

Streamlining every step in microbial identification, Applied Biosystems™ MicroSEQ™ System combines the advantages of MicroSEQ ID Software with easy PrepMan Ultra sample preparation reagents and protocols, MicroSEQ™ bacterial and fungal application kits, and industry-leading thermal cyclers and sequencing systems. To ensure optimal performance and system-wide integration, we develop and test all components of the system together.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodCapillary Electrophoresis
For Use With (Application)Sequencing
For Use With (Equipment)310 Genetic Analyzer, 3100 Genetic Analyzer, 3100-Avant Genetic Analyzer, 3130xl Genetic Analyzer, 3730xl DNA Analyzer, GeneAmp 9700, 3730 DNA Analyzer, 3500xL Genetic Analyzer, 3500 Genetic Analyzer
LibrariesCustomizable, Bacterial Library (> 2000 species)
Product LineMicroSEQ™
Product TypeSequencing Kit
Quantity1 Kit
Sample TypePCR Products
Shipping ConditionDry Ice
SoftwareMicroSEQ™ ID Analysis Software
Target16S rRNA (full gene)
Target Organism ClassBacteria
TechniqueSBT (Sequence-Based Typing)
Workflow StepCycle Sequencing
FormatKit
Unit SizeEach
Contents & Storage
Each kit contains three tubes each of forward and reverse sequencing mix. Sufficient reagent is supplied to perform 15 forward and 15 reverse sequencing reactions of the 16s rRNA gene.

Store at -15°C to -25°C.

Frequently asked questions (FAQs)

Where can I get a copy of the free trial version of the MicrobeBridge Software?

A 60-day-free trial of the MicrobeBridge Software can be downloaded here: www.thermofisher.com/order/catalog/product/A29238

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Software Support Center.

Who do I contact for support for MicrobeBridge Software and for MicrobeNet database issues?

For the MicrobeBridge Software, please contact your local Thermo Fisher Scientific Technical Support (https://www.thermofisher.com/us/en/home/technical-resources/contact-us.html) or email abtechsupport@thermofisher.com.

For MicrobeNet, please contact the CDC directly at microbenet@cdc.gov. This account is actively monitored by the CDC MicrobeNet staff.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Software Support Center.

What are the minimum system requirements for installing the MicrobeBridge Software?

The MicrobeBridge Software has the following recommended hardware requirements: Minimum 1.8 GHz processor, 2 GB RAM, and 20 GB of free hard disk space. MicrobeBridge Software has been tested on Windows 7 32-bit SP1, Windows 7 64-bit SP1, and Windows 10 IoT.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Software Support Center.

Will I lose any data if I upgrade from the MicrobeBridge Software free trial version to the full version?

No data is lost with the MicrobeBridge Software installation of the full version on top of the free trial version.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Software Support Center.

Which input file formats can I use with the MicrobeBridge Software?

Only .ab1 files can be used with the MicrobeBridge Software.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Software Support Center.