7300 Real Time PCR Systems Spectral Calibration Kit
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Applied Biosystems™

7300 Real Time PCR Systems Spectral Calibration Kit

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This easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™/SYBR™ Green I, VIC™/JOE™, and NED™/TAMRA™/ROX™ dyes onRead more
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Catalog NumberQuantity
43491821 Kit
Catalog number 4349182
Price (CNY)
18,252.00
Each
Add to cart
Quantity:
1 Kit
Price (CNY)
18,252.00
Each
Add to cart
This easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™/SYBR™ Green I, VIC™/JOE™, and NED™/TAMRA™/ROX™ dyes on the Applied Biosystems™ 7300 Real-Time PCR System. The Applied Biosystems™ 7300 Real-Time PCR Systems Spectral Calibration Kit I contains one background plate, one region of interest (ROI) calibration plate, and seven spectral calibration plates with seven separate dye standards.

• 96-well optical reaction plates preloaded with dyes (FAM™/SYBR™ Green I, VIC™/JOE™, NED™/TAMRA™/ROX™) enable you to calibrate the 7300 Real-Time PCR System quickly and easily
• Easy-to-use, preloaded spectral calibration plates reduce setup time
• Provides accurate discrimination of the fluorescence signal of individual dyes in the reaction mixture
• Includes the Passive Reference I dye standard for signal normalization in all real-time and endpoint reactions

Spend Your Time on Research, Not Instrument Calibration
This kit is easy-to-use, preloaded, 96-well optical reaction plates enable quick and easy calibration of the 7300 Real-Time PCR System. Insert the reaction plates during system installation, and the system automatically collects the dye standard's spectral information. The system's application algorithm then uses this calibration information to ensure accurate data analysis.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Calibrated DyeFAM, JOE, NED, ROX, TAMRA™, VIC, SYBR™ Green
Calibration TypeSpectral Calibration
Detection MethodFluorescence
For Use With (Application)Real Time PCR (qPCR)
For Use With (Equipment)7300 System
Quantity1 Kit
Shipping ConditionDry Ice
Product TypeSpectral Calibration Kit
Unit SizeEach
Contents & Storage
Contains one background plate, one ROI calibration plate,and seven spectral calibration plates with FAM™⁄SYBR™ Green I, VIC™⁄JOE™, and NED™⁄TAMRA™⁄ROX™ dye standards. Store at -15 to -25°C. Protect from light.

Frequently asked questions (FAQs)

What type of Real-Time PCR (Sequence Detection System) information is available from Applied Biosystems?

Tutorials demonstrating introductions to Real-Time PCR, guidelines for designing real-time assays using Primer Express software, and various instrument set-ups can be found under Life Technologies University on our website. User bulletins, manuals and Product inserts, protocols can be found as downloadable pdf files on our Technical Resources page.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.