7900HT TaqMan™ Low Density Array Instrument Verification RNase P Kit

The 7900HT TaqMan® Low Density Array Instrument Verification RNase P kit is used to validate the performance of the AppliedRead more

Catalog Number	Quantity
4351468	1 kit

Catalog number 4351468

Price (CNY)

31,556.00

Add to cart

Quantity:

1 kit

Price (CNY)

31,556.00

Add to cart

The 7900HT TaqMan® Low Density Array Instrument Verification RNase P kit is used to validate the performance of the Applied Biosystems® 7900HT Fast Real-Time PCR Systems with the TaqMan® Low Density Array Block Upgrade. The 7900HT TaqMan® Low Density Array Instrument Verification RNase P kit contains an empty TaqMan® Low Density Array and 8 vials of RNase P detection reagents to detect and quantitate genomic copies of the human RNase P gene, a single-copy gene encoding the RNA moiety of the RNase P enzyme. Each vial contains premixed reagents (1X TaqMan® Universal PCR Master Mix, RNase P primers, and FAM™ dye–labeled probe) and template. The 7900HT TaqMan® Low Density Array Instrument Verification RNase P kit must demonstrate the ability to distinguish between 5,000 and 10,000 genomic equivalents with a 99.7% confidence level for a subsequent sample run in a single well. To ensure accurate results, Applied Biosystems® recommends that the performance of your instrument be verified every six months.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Calibrated DyeFAM

Calibration TypeRNase P Calibration, Instrument Verification Plate

Detection MethodFluorescence

For Use With (Application)Real Time PCR (qPCR)

For Use With (Equipment)7900 HT LDA System

Product LineTaqMan

Quantity1 kit

Product TypeInstrument Verification Kit

Unit SizeEach

Contents & Storage

Contains an empty TaqMan™ Low Density Array and 8 vials of RNase P detection reagents. Store at -15 to 25°C. Protect from light

Frequently asked questions (FAQs)

When do I need to calibrate the 7900HT Fast Real-Time PCR System?

Recommended Maintenance Schedule

Power on/off the computer controlling the instrument: Weekly
Check computer disk space. If necessary, archive or back up your experiment files and instrument settings: Weekly
Run disk cleanup and disk defragmentation: Every month
Background calibration: Every month
Perform an instrument self test: Every month
Pure dye calibrations: Every 6 months
RNaseP instrument verification: After the instrument has been moved, or as needed to verify instrument performance

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Can I use my 7900HT System RNase P instrument verification plates with both the 7900HT System and the QuantStudio 6 and 7 Flex Real-Time PCR Systems?

Only the TaqMan RNase P Instrument Verification Plate, Fast 96-well (for 0.1 mL block) (Cat. No. 4351979) is compatible with both the 7900HT System and the QuantStudio 6 and 7 Flex Real-Time PCR Systems. The following RNase P instrument verification plates can be used on the 7900HT System, but not on the QuantStudio 6 and 7 Flex Real-Time PCR Systems:
• TaqMan RNase P Instrument Verification Plate, 96-well (Cat. No. 4310982) (for 96-well standard block)
• TaqMan RNase P Instrument Verification Plate for 7900HT System, 384-well (Cat. No. 4323306) (for 384-well block)
• 7900HT TaqMan Low Density Array Instrument Verification RNase P Kit (Cat. No. 4351468) (for TaqMan Array Card block)

The following RNase P instrument verification plates can be used on the QuantStudio 6 and 7 Flex Real-Time PCR Systems, but not on the 7900HT System:
• TaqMan RNase P Instrument Verification Plate for ViiA7 System, 96-well (Cat. No. 4432382) (for 96-well standard block)
• TaqMan RNase P Instrument Verification Plate for ViiA7 System, 384-well (Cat. No. 4455280) (for 384-well block)
• ViiA7 Array Card RNase P Verification Kit (Cat. No. 4432265) (for TaqMan Array Card block)

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What is the difference between a Fast 96-well and standard 96-well block?

There are two main differences between a Fast 96-well and standard 96-well block for the Applied Biosystems real-time PCR systems:
• Reaction well volume: The Fast 96-well blocks have 0.1 mL reaction wells and are compatible with 0.1 mL plates and tubes. The standard 96-well blocks have 0.2 mL reaction wells and are compatible with 0.2 mL plates and tubes.
• Ramp rate: The fast 96-well blocks have a higher maximum ramp rate than standard blocks for the same real-time PCR system. However, both the fast 96-well and standard 96-well blocks are capable of running standard and fast chemistry.

Note: For newer instruments (QuantStudio 3 and 5 Real-Time PCR Systems and later) "0.1 mL" and "0.2 mL" are being used in place of "fast" and "standard" as designations for the two 96-well block formats.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.