StepOne™ Real-Time PCR System Spectral Calibration Kit (48 well)

This easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™/SYBR™ Green I, VIC™/JOE™, and ROX™ dyes onRead more

Catalog Number	Quantity
4371433	1 kit

Catalog number 4371433

Price (CNY)

8,699.00

Add to cart

Quantity:

1 kit

Price (CNY)

8,699.00

Add to cart

This easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™/SYBR™ Green I, VIC™/JOE™, and ROX™ dyes on the StepOne™ Real-Time PCR System.

Features of the StepOne™ Real-Time PCR System Spectral Calibration Kit include:

• 48-well optical reaction plates preloaded with dyes (FAM™/SYBR™ Green I, VIC™/JOE™, and ROX™) are provided for quick and easy calibration of the StepOne™ Real-Time PCR System
• Easy-to-use and provides accurate discrimination of the fluorescence signal of individual dyes in the reaction mixture
• Includes the Passive Reference I dye standard for signal normalization in all real-time and endpoint reactions

The Applied Biosystems™ StepOne™ Real-Time PCR System Spectral Calibration Kit contains one background plate and three preloaded, 48-well fast optical reaction plates containing five dye standards.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Calibrated DyeFAM, JOE, ROX, VIC, SYBR™ Green

Calibration TypeSpectral Calibration

Detection MethodFluorescence

For Use With (Application)Real Time PCR (qPCR)

For Use With (Equipment)StepOne™, Fast Mode, StepOne™, Standard Mode

Product LineStepOne

Quantity1 kit

Shipping ConditionDry Ice

Product TypeSpectral Calibration Kit

Unit SizeEach

Contents & Storage

Contains three 48-well reaction plates—one background plate and two containing dye standards(plates I & II). Plate I contains FAM, JOE and ROX; Plate II contains SYBR™ Green I and VIC.

Store at -15 to 25°C. Protect from light.

Expiration date: 12 months from the date of delivery

Frequently asked questions (FAQs)

What does "Error - Cannot calculate Pure Dye Matrix" mean?

This issue can occur if the run is interrupted. To prevent this from happening in the future, avoid ending the PCR with an infinite hold stage or interrupting the run. Please send the file to techsupport@thermofisher.com so that our software group can attempt to recover the data.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What does "Error - Fatal Error Number 0x8158" mean?

This is generally caused by incorrect placement of tube strips, resulting in a sample block that can't properly be lowered. We recommend contacting Instrument Services to have an engineer walk you through a manual drawer-lowering process during instrument power-up. To prevent this from happening in the future, place tubes toward the middle of the block or use a placeholder strip on the opposing side to counterbalance block pressure.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What does "Error - Java.lang.IllegalArgumentException: One of the raw spectra is null" mean?

This issue can occur if the “Start Run” button is clicked more than once or if illegal characters are used in the experiment/file name. You can recover the data yourself by overriding the calibration file with one that represents the calibration status of the instrument when you ran that particular file. Use the toolbar at the top to navigate: Analysis then Override Calibration then Use Calibration From Another File.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What does "Error - Cannot open the file because file contents are not recognized" mean?

This issue can occur if duplicate samples are added to the experiment, if illegal characters are used in the experiment/file name, or if the file extension was changed to .eds from a different extension.
Please send the file to techsupport@thermofisher.com to attempt recovery.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What does "Error - Analysis failed due to GExSession doesn't exist in context" mean?

This error means that the data collection was not turned on as appropriate. If this is inadvertently turned off, no data will be collected. Thus, the data are not retrievable in such a file.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

View all StepOne™ Real-Time PCR System Spectral Calibration Kit (48 well) FAQs