Fast SYBR™ Green Master Mix
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Applied Biosystems™

Fast SYBR™ Green Master Mix

Fast SYBR™ Green Master Mix Real-Time PCR Master Mix Designed for Speed Obtain a fast, reliable, and cost-effective solution forRead more
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Catalog NumberQuantity
43856101 x 1 mL
43856121 x 5 mL
43856162 x 5 mL
43856175 x 5 mL
438561810 x 5 mL
43856141 x 50 mL
Catalog number 4385610
Price (CNY)
1,302.00
Each
Add to cart
Quantity:
1 x 1 mL
Price (CNY)
1,302.00
Each
Add to cart
Fast SYBR™ Green Master Mix Real-Time PCR Master Mix Designed for Speed Obtain a fast, reliable, and cost-effective solution for your real-time PCR applications without compromising sensitivity, specificity, dynamic range, or PCR efficiency.
• Fast—Real-time PCR results in as fast as 35 minutes
• Sensitive—Detect very low copies of target
• Specific—Minimize primer-dimer and non-specific amplification
• Reproducible—Consistent amplification across a wide dynamic range Fast SYBR™ Green Master Mix contains all of the components, excluding the template and primers, in a convenient 2X master mix. It includes the following components in an optimized buffer:
• AmpliTaq™ Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start, minimizing non-specific product formation and enabling reactions to be set up at room temperature
• SYBR™ Green I dye to enable detection of double-stranded DNA
• Deoxynucleotides (dNTPs) to help maintain optimal PCR results
• Uracil-DNA Glycosylase (UDG) designed to reduce carryover contamination
• Passive internal reference based on proprietary ROX™ dye to enable increased precision

Alternative product
Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Fast SYBR Green Master Mix and added additional capabilities for your gene expression analysis.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Equipment)7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
FormatTube
No. of Reactions100 Reactions
Passive Reference DyeROX (Pre-mixed)
PolymeraseAmpliTaq Fast DNA Polymerase, AmpliTaq Fast UP
Product LineSYBR
Product TypeReal Time PCR SYBR Master Mix
Purity or Quality GradeUP (Ultra Pure)
Quantity1 x 1 mL
Sample TypeDNA (Genomic), cDNA
Shipping ConditionDry Ice
Sufficient For100 Reactions of 20 μL
Volume1 mL
Concentration2X
Detection MethodSYBR
For Use With (Application)Gene Expression
GC-Rich PCR PerformanceHigh
Label or DyeSYBR Green
PCR MethodqPCR
Reaction SpeedFast
Unit SizeEach
Contents & Storage
1 x 1 mL vial. The 2X mix contains SYBR™ Green 1 Dye, AmpliTaq Gold™ Fast DNA Polymerase LD, dNTPs with dUTP⁄dTTP blend, Passive Reference 1 and optimized buffer components. 100 reactions in a 20 μL total volume. Store at -5 to -30°C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

Frequently asked questions (FAQs)

Is there a SYBR Green Fast Master Mix currently available?

Yes. The following reagents can be used for fast cycling: Fast SYBR Green Master Mix, EXPRESS SYBR GreenER qPCR Supermix Universal, and SYBR GreenER qPCR SuperMix for ABI PRISM instruments.

Citations & References (1)

Citations & References
Abstract
Decrease of miR-146b-5p in monocytes during obesity is associated with loss of the anti-inflammatory but not insulin signaling action of adiponectin.
Authors:Hulsmans M, Van Dooren E, Mathieu C, Holvoet P,
Journal:PLoS One
PubMed ID:22393448
Low adiponectin, a well-recognized antidiabetic adipokine, has been associated with obesity-related inflammation, oxidative stress and insulin resistance. Globular adiponectin is an important regulator of the interleukin-1 receptor-associated kinase (IRAK)/NF?B pathway in monocytes of obese subjects. It protects against inflammation and oxidative stress by inducing IRAK3. microRNA (miR)-146b-5p inhibits NF?B-mediated inflammation ... More