TaqMan™ Cells-to-CT™ Control Kit
TaqMan&trade; Cells-to-C<sub>T</sub>&trade; Control Kit
Invitrogen™

TaqMan™ Cells-to-CT™ Control Kit

Green features
The Ambion™ TaqMan™ Cells-to-CT Control Kit is designed for use with the TaqMan™ Gene Expression Cells-to-CT Kit. The Control KitRead more
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Catalog NumberQuantity
4386995100 reactions
Catalog number 4386995
Price (CNY)
10,188.00
Each
Add to cart
Quantity:
100 reactions
Price (CNY)
10,188.00
Each
Add to cart
The Ambion™ TaqMan™ Cells-to-CT Control Kit is designed for use with the TaqMan™ Gene Expression Cells-to-CT Kit. The Control Kit includes XenoRNA™ Control, a synthetic RNA transcript with a unique sequence that lacks homology to current annotated biological sequences, making it an ideal control for any experiment. The Control Kit also includes a TaqMan™ Gene Expression Assay for the XenoRNA™ Control target, and a TaqMan™ Gene Expression Assay for the highly expressed endogenous control gene β-actin.

Features of the The Ambion™ TaqMan™ Cells-to-CT Control Kit:
• Confirms sufficient cell input
• Monitors the presence of RT or real-time PCR inhibitors
• Controls for sample normalization

Monitor Inhibition and Confirm Sufficient Cell Input
The TaqMan™ Cells-to-CT™ Control Kit is used to assess inhibition and cell input in Cells-to-CT reactions. For example, the XenoRNA™ Control confirms that samples can support RT-PCR and is an indicator of reaction inhibitors. By adding the XenoRNA™ Control to the Stop Solution during the last step of the cell lysis procedure, each sample is provided with a constant amount of control target and subsequently amplified by the XenoRNA™ TaqMan™ Gene Expression Assay.

Amplification with the β-actin Gene Expression Assay functions as an endogenous control for normalization, and ensures that the lysis reaction contains a sufficient number of cells—this is important for experiments with fewer than ∼100 cells and monitors the effectiveness of cell lysis.

Evaluate Compatibility with New Cell Lines
The TaqMan™ Cells-to-CT™ Control Kit is ideal for assessing the compatibility of untested cell lines with the TaqMan™ Gene Expression Cells-to-CT Kit to determine the maximum number of cells that can be used per lysis reaction.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Control TemplateXenoRNA™ control (synthetic RNA transcript)
FormatTube
Gene SymbolACTB
Green FeaturesFewer resources used and less waste, Less hazardous
Internal Probe ModificationMGB (Minor Groove Binder) (3'), Non-Fluorescent Quencher (3'), FAM (5')
No. of Reactions100 Reactions
Product LineAmbion™, Cells-to-CT™, TaqMan™
Product TypeCells-to-C Control Kit
Quantity100 reactions
Shipping ConditionDry Ice
Sufficient For100 Reactions
TargetXenoRNA™ control, β-actin
Detection MethodPrimer-probe
For Use With (Application)Gene Expression
GC-Rich PCR PerformanceHigh
Label or DyeFAM
PCR MethodqPCR
Unit SizeEach
Contents & Storage
Xeno™ RNA Control, 20X Xeno™ RNA Control Taqman™ Gene Expression Assay, and 20X ACTB (β-actin) TaqMan™ Gene Expression Assay are all stored at -20°C.

All reagents are guaranteed stable for 6 months when properly stored.

Frequently asked questions (FAQs)

I'm seeing PCR products in the minus-RT control after performing my Cells-to-CT experiment. What does this mean?

If PCR products are seen in the minus-RT control reaction, but not in the no-template control, it indicates that genomic DNA remains in the sample and that genomic DNA was amplified in real-time PCR. Please follow the suggestions below:

- Ensure the DNase I is mixed thoroughly into the Lysis Solution.
- Use fewer cells per lysis reaction.
- Lyse cells using Lysis Solution that is at room temperature, and make sure that the lysis reaction occurs at room temperature.
You can also try increasing the incubation time of the lysis reaction to 8 minutes and/or using Lysis Solution that has been warmed up to 25 degrees C for cell lysis.

I'm getting PCR products in the no-template PCR control when performing a Cells-to-CT experiment. What could cause this?

PCR products in the no-template PCR control indicate that the sample is contaminated with DNA. More stringent steps need to be taken to control contamination.

I'm getting no PCR product or unexpected PCR products after performing a Cells-to-CT experiment. What could be the cause of this?

Please review the following possibilities and suggestions:

- A problem with adding or mixing the Stop Solution: ensure that the Stop Solution was added directly to the lysate, as components of the Lysis Solution may inhibit RT-PCR if not fully inactivated.
- The RNA was degraded: keep cells in PBS on ice before starting the cell lysis procedure.
- RNase in the sample was not completely inactivated: Too many cells could have been used or too much PBS left on the cells, diluting the lysis solution.
- The lysates sat too long before going to room temperature: Do not allow lysates to sit longer than 20 minutes at room temperature once the Stop Solution has been added.
- The sample does not contain the target RNA: Verify that the procedure is working by using the XenoRNA Control in the sample. Also check that your PCR primers can amplify your target under the PCR conditions you are using.

I ran out of stop solution for my Cells-to-CT experiment. Can I purchase it separately?

Yes, it is available in 1 mL aliquots (Cat. No. 4402960).

I have genomic DNA contamination in my Cells-to-CT reaction. How do I get rid of it?

1. Ensure that all medium is removed from the wells.
2. Wash with an equal volume of room temperature 1X PBS after the medium is removed.
3. Ensure that the reaction happens at room temperature (the lysis reaction may not reach room temperature if the plate is on ice, if the plate was quickly moved to the bench, or if a cold lysis solution was added).
4. Warm lysis solution to room temperature before adding to cells.
5. Allow the lysis reaction to proceed for 8 minutes at 25 degrees C.

View all TaqMan™ Cells-to-CT™ Control Kit FAQs