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Applied Biosystems™
Power SYBR™ Green RNA-to-CT™ 1-Step Kit
The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.
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| Catalog Number | No. of Reactions |
|---|---|
| 4389986 | 500 Reactions |
| 4391178 | 100 Reactions |
Catalog number 4389986
Price (CNY)
9,665.00
Each
No. of Reactions:
500 Reactions
Price (CNY)
9,665.00
Each
The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.
Get sensitivity and specificity in an easy-to-use one-step qRT-PCR reaction. The Power SYBR™ Green RNA-to-CT™ 1-Step kit has been formulated to ensure maximum sensitivity and reliability. The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets. It includes:
- AmpliTaq™ Gold DNA Polymerase UP for hot start qPCR, minimizing non-specific product formation
- ArrayScript™ UP Reverse Transcriptase, an engineered RT that produces high yields of full-length cDNA
- An additive that reduces primer dimer formation, reducing non-specific products that can cause false positive signals
- RNase inhibitor, to prevent degradation of RNA templates
- A proprietary ROX™ dye that serves as a passive internal reference to normalize non-PCR-related fluorescence fluctuations for superb precision on Applied Biosystems™ real-time PCR instruments.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Equipment)7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System
No. of Reactions500 Reactions
Passive Reference DyeROX (Pre-mixed)
PolymeraseAmpliTaq Gold DNA Polymerase
Product LineRNA-to-CT™, SYBR™
Product TypeOne-Step qRT-PCR Kit
Purity or Quality GradeUP (Ultra Pure)
Quantity500 Reactions
Reverse TranscriptaseArrayScript™ UP
Run TimeStandard
Sample TypeRNA
Shipping ConditionWet Ice
Sufficient For500 Reactions at 20 μL
Detection MethodSYBR
For Use With (Application)Gene Expression
Label or DyeSYBR Green
PCR Method1-step RT-qPCR
Reaction SpeedStandard
Unit SizeEach
Contents & Storage
Sufficient for 500 reactions at 20 μL reaction volume and includes:
• 1 x 5 mL tube of 2X master mix containing SYBR™Green 1 Dye, AmpliTaq Gold™ DNA Polymerase UP, dNTPs, Passive Reference 1,and optimized buffer components. Store at 2-8°C after opening.
• 1 x 80 μL tube of 125X RT enzyme mix containing ArrayScript™ UP Reverse Transcriptase, RNase Inhibitor. Store at -15 to -30°C.
Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
• 1 x 5 mL tube of 2X master mix containing SYBR™Green 1 Dye, AmpliTaq Gold™ DNA Polymerase UP, dNTPs, Passive Reference 1,and optimized buffer components. Store at 2-8°C after opening.
• 1 x 80 μL tube of 125X RT enzyme mix containing ArrayScript™ UP Reverse Transcriptase, RNase Inhibitor. Store at -15 to -30°C.
Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).