AmpliTaq Gold™ Fast PCR Master Mix
AmpliTaq Gold™ Fast PCR Master Mix
Applied Biosystems™

AmpliTaq Gold™ Fast PCR Master Mix

The Applied Biosystems AmpliTaq Gold Fast PCR Master Mix is a premix, hot start chemistry for end-point PCR analysis.
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Catalog NumberNo. of Reactions
4390939250 Reactions
Catalog number 4390939
Price (CNY)
2,101.00
Each
Add to cart
No. of Reactions:
250 Reactions
Request bulk or custom format
Price (CNY)
2,101.00
Each
Add to cart
The Applied Biosystems™ AmpliTaq Gold™ Fast PCR Master Mix is a premix, hot start chemistry for end-point PCR analysis. Its optimized chemical design enables to shorten PCR running time from a traditional 2 hours to proximal 40 minutes. It generates high quality PCR products that give high quality sequencing results that customers expect from Applied Biosystems™. Optimized for use in sequencing applications, this kit provides specific, high-yield amplicons that can be easily and robustly sequenced. This premixed master mix works with your existing primers to provide sensitive, specific and reproducible results.
  • Fast Results—Speeds the time from sample to results. Amplifies 600bp length gene in approximately 45 minutes
  • Better Sequencing quality—Specific, high-yield amplicons suitable for sequencing applications that yield low peak-under-peak, longer continous read-length and higher QV-30 count
  • Easy to use—The 2X master mix allows for quick experiment setup
  • No primer annealing restrictions

Speed Time to Discovery

AmpliTaq Gold™ Fast PCR Master Mix, UP brings PCR reaction times down to as little as 45 minutes and delivers robust sequencing data on par with standard PCR reagents such as the classic AmpliTaq Gold™ PCR Master Mix. This new master mix has been designed for use with your existing primers and results in specific, high-yield amplicons for easy, high quality sequencing data. Cycle sequencing can now also be performed in as little as 50 minutes.

Quick Setup, High Sensitivity

This pre-mixed hotstart kit allows you to setup your experiment quickly without sacrificing performance. This kit provides reproducible, sensitive results, allowing you to detect a single copy of a gene in 10 ng of DNA.

Robust Fast Sequencing Results

Get the sequencing results you expect from Applied Biosystems™. The AmpliTaq Gold™ Fast PCR Master Mix, UP is optimized for your sequencing reactions. This kit yields specific high-yield amplicons that provide you with clean sequencing reads including low peak-under-peak, longer read-lengths and superior Q30.

Note: See user's manual or package insert for limited label license, and trademark information.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Exonuclease Activity5' - 3'
Fidelity (vs. Taq)1X
FormatTube
Hot StartBuilt-In Hot Start
No. of Reactions250 Reactions
Overhang3'-A
PolymeraseAmpliTaq Gold DNA Polymerase
Product TypeFast PCR Master Mix
Quantity250 reactions
Reaction FormatSuperMix or Master Mix
Shipping ConditionWet Ice
Size (Final Product)1.5 kb or less
Starting MaterialDNA
Volume2.5 mL
Concentration2X
For Use With (Application)Cloning, Hot-start PCR
GC-Rich PCR PerformanceHigh
Reaction SpeedFast
Unit SizeEach
Contents & Storage
• AmpliTaq Gold Fast PCR Master Mix, 2 x 1.25 mL tubes

Store at 2°C to 8°C.

Frequently asked questions (FAQs)

What is the difference between AmpliTaq Gold polymerase and Platinum Taq polymerase?

Both AmpliTaq Gold and Platinum Taq are hot-start enzymes that allow you to set up your reactions on the benchtop without the need for ice. AmpliTaq Gold is a chemically-modified hot-start enzyme, provided in an inactive state. Heat activates the enzyme, with full activity after 10 min at 95 degrees C. Platinum Taq is an antibody-mediated hot-start enzyme. The anti-Taq antibodies bind and inactivate the enzyme, until the heat denaturation step of the PCR reaction (30 sec to 2 min), which activates the enzyme.

When using AmpliTaq Gold DNA polymerase, under what conditions would one choose a two-temperature vs. three-temperature PCR?

A two-temperature PCR is commonly used when the primer annealing temperatures are above 60 degrees C. Use a three-temperature PCR when the templates have high G+C content and/or secondary structure, or desired primer annealing temperatures are below 60 degrees C. Please consult the Product Insert for more information on the use of AmpliTaq Gold DNA polymerase.

Is there anything to prevent AmpliTaq Gold DNA polymerase from extending from the 3’ end of a TaqMan probe in a 5’ nuclease assay?

Yes. There is a phosphate group on the 3' end of all TaqMan probes that prevents such extension.

How does AmpliTaq Gold DNA Polymerase differ from AmpliTaq DNA Polymerase?

AmpliTaq Gold DNA Polymerase is a modified form of AmpliTaq DNA Polymerase that contains a proprietary chemical (or so-called hot start molecule) bound to the enzyme's active site. In order to activate the AmpliTaq Gold DNA Polymerase fully, we recommend an initial activation step of 95 degrees C for 10 min when using GeneAmp 10X PCR Buffer I and/or GeneAmp 10X PCR Buffer II and Mg in one of our thermal cyclers. When using GeneAmp 10X PCR Gold Buffer, activation time can be reduced to 5 minutes. Once activation is complete, you can proceed with your standard PCR cycling program (denaturing, annealing, extension, etc).

Does AmpliTaq Gold DNA Polymerase contain exonuclease (proofreading) activity?

No, AmpliTaq Gold DNA polymerase does not contain proofreading activity, however fidelity in PCR amplifications utilizing this enzyme may be improved. High fidelity can be achieved by: 1. Decreasing the final concentration of each nucleotide to 40-50 uM. 2. Using the lowest MgCl2 concentration possible. 3. Using less enzyme. 4. Decreasing extension times. 5. Using the highest annealing temperature possible. 6. Using as few cycles as possible.

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