Megaplex™ PreAmp Primers, Rodent Pool A
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Applied Biosystems™

Megaplex™ PreAmp Primers, Rodent Pool A

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For use when assay sensitivity is of the utmost importance, Applied Biosystems® Megaplex™ PreAmp Primers significantly enhance the ability toRead more
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Catalog NumberQuantity
439920350 reactions
Catalog number 4399203
Price (CNY)
11,934.00
Each
Add to cart
Quantity:
50 reactions
Price (CNY)
11,934.00
Each
Add to cart
For use when assay sensitivity is of the utmost importance, Applied Biosystems® Megaplex™ PreAmp Primers significantly enhance the ability to detect low expressed microRNAs enabling the generation of a comprehensive expression profile using far less sample, as low as 1 ng of input total RNA. With content matched to the Megaplex™ RT Primers, Megaplex™ PreAmp Primers, Rodent Pool A is designed to be used with Megaplex™ RT Primers, Rodent Pool A. Following reverse transcription using Megaplex™ RT Primers, Megaplex™ PreAmp Primers along with the TaqMan® PreAmp Master Mix uniformly amplify all microRNAs prior to quantitation by real-time PCR using TaqMan® MicroRNA Arrays.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Fidelity (vs. Taq)10 X
Green FeaturesSustainable packaging
No. of Reactions50 reactions
PrimerPreAmp Primers
Product LineMegaplex™, TaqMan™
Product TypePreamp Primer
Quantity50 reactions
Shipping ConditionRoom Temperature
SpeciesRat, Mouse
Sufficient For50 Reactions
Concentration10X
GC-Rich PCR PerformanceLow
PCR MethodqPCR
Unit SizeEach
Contents & Storage
Contains 150 μL of 10X primers

Store at -15° to -25°C.

Frequently asked questions (FAQs)

Why does the negative control well show amplification when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the reagents or the cDNA template are contaminated. Please follow established PCR laboratory best practices.

Why do I have poor reproducibility across technical replicates when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the reagents were not adequately mixed. Ensure that all samples and reagents are mixed well.

When doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards, why are the Ct values for the endogenous controls highly variable across the sample set?

Highly variable values for endogenous controls is most likely due to biological variation. We recommend that you use an alternative endogenous control, such as a non-variable miRNA.

Why is the Ct value for the no-template control (NTC) <35 for some TaqMan microRNA Assays?

The Ct value for the NTC can be <35 for the following reasons:
- There are non-specific interactions between primers. For NTC information on a specific assay, see the Megaplex Assay Performance File.
- The cDNA template is contaminated. Please ollow established PCR good laboratory practices to prevent contamination.
- The preamplification product was not diluted properly before real-time PCR. Ensure that you dilute the preamplification product according the procedure in the user guide.

Why can I not detect any miRNA in my sample using the TaqMan MicroRNA Assay?

Most likely, the threshold is set too high to detect miRNA in samples with low levels of expression. We recommend adjusting the threshold (Ct) or NOAMP flag threshold (Crt) to an appropriate level.

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