TaqMan™ OpenArray™ Genotyping Master Mix

Applied Biosystems™

TaqMan™ OpenArray™ Genotyping Master Mix

This 2X concentrated mix is optimized for SNP genotyping using TaqMan™ OpenArray™ SNP Genotyping Plates.For Research Use Only. Not forRead more

Catalog Number	Quantity
4404846	1 kit

Catalog number 4404846

Price (CNY)

6,661.00

Add to cart

Quantity:

1 kit

Price (CNY)

6,661.00

Add to cart

This 2X concentrated mix is optimized for SNP genotyping using TaqMan™ OpenArray™ SNP Genotyping Plates.

For Research Use Only. Not for use in diagnostics procedures.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Fidelity (vs. Taq)2 X

For Use With (Equipment)OpenArray™

Genotyping TargetDrug Metabolizing Enzymes, SNPs (Known)

Hot StartBuilt-In Hot Start

Passive Reference DyeROX (Pre-mixed)

PolymeraseAmpliTaq Gold DNA Polymerase

Product LineOpenArray™, TaqMan™

Product TypeGenotyping Master Mix

Quantity1 kit

Sample TypeDNA

Shipping ConditionWet Ice

TechniqueSNP Genotyping (Real-Time PCR-Based)

Concentration2X

Detection MethodPrimer-probe

For Use With (Application)Genotyping

PCR MethodqPCR

Reaction SpeedStandard

Unit SizeEach

Contents & Storage

Includes TaqMan™ OpenArray™ Genotyping Master Mix, with AmpliTaq Gold™ DNA Polymerase UP (Ultra Pure), dNTPs, and optimized mix components. This pack contains one 5 mL bottle, sufficient for 32 plates. Store at 2–8°C.

Frequently asked questions (FAQs)

What are the cycling conditions for OpenArray off-line cycling on a ProFlex PCR System?

These instructions may be found on page 79 of the ProFlex PCR System User Guide (https://tools.thermofisher.com/content/sfs/manuals/MAN0007697.pdf).

Reaction volume: 33 nL
Cover temperature: 70°C

94°C for 600 sec, ramp rate 1.3

50 cycles of:
99°C for 30 seconds, ramp rate 1.3
54°C for 21 seconds, ramp rate 1.1
57°C for 90 seconds, ramp rate 0.1

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What are the cycling conditions for OpenArray off-line cycling on a GeneAmp PCR System 9700?

These instructions may be found on page 5 of the Dual Flat Block GeneAmp PCR System 9700 User Guide (https://tools.thermofisher.com/content/sfs/manuals/cms_065075.pdf).

93°C for 10 min, ramp rate 100%

50 cycles of:
95°C for 45 sec, 84% ramp rate
94°C for 13 sec, 100% ramp rate
53°C for 2 min 14 sec, 44% ramp rate

25°C for 2 min, 100% ramp rate

Optional: Add a post-PCR overnight hold as shown below:
4°C for 99 min 59 sec, 100% ramp rate

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What additional accessories do I need to perform off-line cycling for QuantStudio OpenArray plates?

You need an OpenArray Plate Adaptor, Cat. No. 4472306. You do not need a compression pad. This part number works with both the GeneAmp PCR System 9700 and the ProFlex PCR System.
Please note that this part number does not have a product page on our website. To order, please call our customer service team at 800-955-6288, option 1, option 1.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

How can I get additional throughput for the QuantStudio 12K Flex OpenArray System?

Additional OpenArray plates can be run off-line using a flat-block thermal cycler. The two thermal cyclers we recommend are the GeneAmp PCR System 9700 and the ProFlex PCR System.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.