TaqMan™ Array, Human Stem Cell Pluripotency, Fast 96-well
TaqMan™ Array, Human Stem Cell Pluripotency, Fast 96-well
Applied Biosystems™

TaqMan™ Array, Human Stem Cell Pluripotency, Fast 96-well

These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, orRead more
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Catalog NumberQuantity
44187221 x 96-well plate
Catalog number 4418722
Price (CNY)
2,663.00
Each
Add to cart
Quantity:
1 x 96-well plate
Price (CNY)
2,663.00
Each
Add to cart
These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The panel of assays in the TaqMan® Array 96-well Human Stem Cell Pluripotency Plate was selected in cooperation with the International Stem Cell Initiative (2007 Nature Biotechnology. 25:803-816). The assays target genes which are validated as markers for the characterization of human embryonic stem cell identity, and which assess variations between embryonic stem cell isolates. Genes are included based on the following criteria: 1) Expression in human embryonal carcinoma cells, primordial germ cells or human embryonic stem cells; 2) Known role in maintenance of pluripotency in human embryonic stem cells; 3) Identification in microarray-based experiments as being correlated with the stem cell state. In order to identify cells which have left the pluripotent state, we have also included genes whose expression is characteristic of specific differentiation lineages in the post implantation embryo and mature tissues.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormDried Down
IncludesTaqMan Assay for One 10 uL Reaction
Internal Probe ModificationMGB (Minor Groove Binder) (3'), Non-Fluorescent Quencher (3'), FAM (5')
Label or DyeFAM
Product LineTaqMan™
Quantity1 x 96-well plate
Reaction SpeedFast
Research CategoryStem Cell Research, Reproduction, Development
TypeArray Plate
Format96-array Plate
SpeciesHuman
Unit SizeEach
Contents & Storage
Each TaqMan™ Array Plate well contains a single TaqMan™ Assay for one 10 μL reaction. The plates can be stored at room temperature or at 4°C.

Frequently asked questions (FAQs)

What mastermix can I use for running samples on TaqMan Gene Signature Array Plates?

We recommend that for standard (20 µL) TaqMan Array Plates, you use either the Universal Master Mix (I or II) or the Gene Expression MasterMix. For fast (10 µL) TaqMan Array Plates, use the Fast Universal Master Mix. The Fast Advanced Master Mix can also be used for either plate type, but we recommend following the protocol in this manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0025706_TaqManFastAdvMM_UG.pdf).

What is the minimum amount of TaqMan array plates I need to purchase to submit an order?

The minimum amount of TaqMan Array plates needed to submit an order is based on the plate type:
• Inventoried TaqMan array plates: 1 plate
• TaqMan Flexible Content panel array plates: 6 plates
• Custom TaqMan array plates: 6 plates
• Specialty TaqMan array plates: 10-20 plates (depends on assay format and configuration). Please contact our Specialty Plates team at Specialty_Plates@thermofisher.com for details.

Can our TaqMan Array Plates be run on non-Applied Biosystem real-time PCR instruments?

We recommend using our Plastics Selection Tool to verify which plates are compatible with your instrument model, https://www.thermofisher.com/us/en/home/life-science/pcr/pcr-plastics/plastics-selection-guide.html.

Based on our testing, the 96-well TaqMan Array Plates can be used on the following non-Applied Biosystem real-time PCR instruments:
• Standard (0.2mL) 96-well Plates: Bio-Rad IQ5
• Fast (0.1mL) Plates: Bio-Rad CFX Connect and Bio-Rad CFX96 Touch

How do I obtain TaqMan documentation files for my TaqMan array plates?

Beginning in April 2016, TaqMan documentation files for TaqMan Array Plates (assay information files (AIF), experimental setup files, and plate layout files) can be downloaded from the Thermo Fisher Scientific website at https://www.thermofisher.com/taqmanfiles. To obtain your TaqMan documentation files:
• Select the appropriate product type.
• Enter the information being requested for the product type.
• Fixed TaqMan array plates: Enter the Part number and Batch number
• TaqMan Flexible Content panel array plates/Custom TaqMan array plates: Enter the Sales Order number and Batch number
• Click Submit.