ViiA™ 7 Array Card Spectral Calibration Kit

This kit is used to perform spectral calibration, region-of-interest (ROI) calibration, and normalization on your ViiA™ 7, QuantStudio™ 7 Flex,Read more

Catalog Number	Quantity
4432314	1 kit

Catalog number 4432314

Price (CNY)

Quantity:

1 kit

This kit is used to perform spectral calibration, region-of-interest (ROI) calibration, and normalization on your ViiA™ 7, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex system with array card block. It includes empty array cards and seven tubes of calibration dyes including: FAM™ dye, VIC™ dye, ROX™ dye, ROI dye mix, background buffer, FAM™⁄ROX™ dye, VIC™⁄ROX™ dye. Please see your instrument maintenance guide for full instructions on use.

The dye formulations for your ViiA™ 7, QuantStudio™ 7, or QuantStudio™ 12K Flex system will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Calibrated DyeFAM, ROX, VIC

Calibration TypeSpectral Calibration, Region-of-Interest (ROI) Calibration, Normalization

For Use With (Application)Real Time PCR (qPCR)

For Use With (Equipment)QuantStudio™ 12 K Flex, QuantStudio™ 7, ViiA™ 7 System

Format384-well Microfluidic Card

Product LineViiA

Quantity1 kit

Product TypeArray Card Spectral Calibration Kit

Unit SizeEach

Contents & Storage

Store 7 Tubes of Calibration Dyes at -15°C to -25°C

Frequently asked questions (FAQs)

I cannot open the QuantStudio 3/5 Design & Analysis 2 (DA2) software on my Mac. What are the software requirements for Mac?

To open the software download on your Mac, you will need to adjust your security settings using the following instructions from Apple support:

Safely open apps on your mac

Please note, the software should run on Mac OS 10.10 or later.

Find additional tips, troubleshooting help, and resources within our QuantStudio 3 and QuantStudio 5 Real Time PCR Systems Support Center.

Can I import a standard curve for my real-time PCR data into the Design & Analysis Software (v2.x)?

It is possible to import a standard curve into the Design & Analysis Software (v2.x) if you are analyzing results from one of the following Applied Biosystems real-time PCR instruments:
• 7900HT Fast Real-Time PCR System
• 7500/7500 Fast Real-Time PCR System
• StepOne/StepOne Plus Real-Time PCR System
• ViiA 7 Real Time PCR System
• QuantStudio 1 Real-Time PCR System
• QuantStudio 3 & 5 Real-Time PCR System
• QuantStudio 6 & 7 Flex Real-Time PCR System
• QuantStudio 6 & 7 Pro Real-Time PCR Systems
• QuantStudio 12K Flex Real-Time PCR system

Follow the instructions below to import a standard curve for analysis in the Design & Analysis Software (v2.x):
1. Open the data file in the Design & Analysis Software (v2.x).
2. Click on Actions.
3. Click on "Standard Curve Analysis Setting".
4. Select "External Standard Curves" and click on "Import".
5. Browse to the proper .csv file and click "Open".
6. Click "Apply".

Can I import a standard curve for my real-time PCR data into the QuantStudio Real-Time PCR Software?

If you are analyzing results from the ViiA 7 Real-Time PCR System, QuantStudio 6 Flex Real-Time PCR System, or QuantStudio 7 Flex Real-Time PCR System, it is possible to import a standard curve into the QuantStudio Real-Time PCR Software following the instructions below:
1. Open the data file in the QuantStudio Real-Time PCR Software.
2. Go to Analysis Settings.
3. Click on the "Standard Curve Settings" tab.
4. Select the option to "Use a standard curve imported from another experiment" and click on "Import".
5. Browse to and highlight the proper data file and click "Select".
6. Click Apply Analysis Settings.

How can I get a summary of the calibrations performed on the QuantStudio 6 and 7 Flex Real-Time PCR Systems?

You can get a summary of the calibrations from the QuantStudio Software by going to Instrument > Instrument Console > Manage Instrument > Information > Print Calibration Log.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Do I need to back up my calibration files before I upgrade the software for the ViiA7 Real-Time PCR System?

No, you do not need to back up the calibration files as all of the calibration files are stored on the ViiA7 Real-Time PCR instrument. Once the computer software is properly installed and the computer is connected to the instrument, the calibration information will automatically update in the QuantStudio Software.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

View all ViiA™ 7 Array Card Spectral Calibration Kit FAQs