Megaplex™ Primer Pools, Human Pools B v3.0

Megaplex™ Primer Pools, Human Pools B v3.0 is available under a single part number containing both the Megaplex™ RT PrimersRead more

Catalog Number	Quantity
4444749	50 reactions

Catalog number 4444749

Price (CNY)

17,261.00

Add to cart

Quantity:

50 reactions

Price (CNY)

17,261.00

Add to cart

Megaplex™ Primer Pools, Human Pools B v3.0 is available under a single part number containing both the Megaplex™ RT Primers and Megaplex™ PreAmp Primers, Human Pools B v3.0. Megaplex™ RT Primers, Human Pool B v3.0 contains RT primers for 377 unique microRNAs and 4 controls. For use when assay sensitivity of the utmost importance, Megaplex™ PreAmp Primers significantly enhance the ability to detect low expressed microRNAs enabling the generation of a comprehensive expression profile using far less sample, as low as 1 ng of input total RNA. The microRNA targets represented in this pool tend be less well characterized, more narrowly expressed and⁄or expressed at a lower level compared to Human Pool A. Megaplex™ Primer Pools, Human Pools B v3.0 are intended for use with the TaqMan® Array Human MicroRNA B Card v3.0, however, they can also be used to prepare cDNA for real-time analysis using the individual TaqMan® MicroRNA Assays.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Green FeaturesSustainable packaging

No. of Reactions50 Reactions

PrimerRT Primers, PreAmp Primers

Product LineMegaplex™

Product TypePrimer Pool

Quantity50 reactions

SpeciesHuman

Sufficient For50 Reactions

For Use With (Application)miRNA analysis

GC-Rich PCR PerformanceLow

PCR MethodqPCR

Unit SizeEach

Contents & Storage

Store at -20°C

Frequently asked questions (FAQs)

Can Ct's greater than a cut-off be considered valid results?

Yes they can. However, it is important to recognize the true linearity and detection limits of your assay: Ct values above the cut-off can indicate non-specific amplification, unless your NTC is a true- no-target control, and you have run a statistically significant number of replicates. Any results with Ct above the recommended cut-off need to be validated with individual assays on plates.

What is a good Ct cut-off for the TaqMan MicroRNA Array Cards and TaqMan Advanced miRNA Array Cards? In other words, beyond what Ct should I not trust the data?

The typical Ct cut-off on TaqMan Array Cards is 32, which is equivalent to Ct 35 on a plate (10 µl reaction). Previous studies show that if you use pre-amplification, a Ct cut-off of 29 or 30 can be used to reduce numbers of false positives (see Technical Note Optimized protocols for human or rodent microRNA profiling with precious samples). To ensure that you have selected a correct cut-off, you should run replicates of the same sample and use Ct cut-off before you see an increase in the Standard Deviation.