TaqMan™ Gene Expression Assay, VIC
TaqMan™ Gene Expression Assay, VIC
Applied Biosystems™

TaqMan™ Gene Expression Assay, VIC

Green features
Applied Biosystems TaqMan Gene Expression assays, VIC, are used for quantitative real-time PCR analysis of gene expression and consist ofRead more
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Catalog NumberQuantity
4448489S (360 reactions), made to order
4448490M (750 reactions), made to order
4448491L (2900 reactions), made to order
Catalog number 4448489
Price (CNY)
4,510.00
Each
Order now
Quantity:
S (360 reactions), made to order

Applied Biosystems TaqMan Gene Expression assays, VIC, are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (VIC) on the 5' end and a minor groove binder (MGB) and nonfluorescent quencher (NFQ) on the 3' end.

Features include:

  • Easy to use—just add cDNA and master mix and run the qPCR—no melt curves required
  • Specific—TaqMan assays use proprietary MGB-containing probes that can be up to 15 bases shorter than non-MGB probes, improving the specificity of the assay
  • Sensitive—TaqMan assays are ideal for measuring low levels of expression or low-abundance targets
  • Accurate—identify small fold-changes with high accuracy of quantitation
  • Extensive content—over 1.8 million predesigned assays available for over 25 different species
  • Gold-standard TaqMan qPCR chemistry—TaqMan assays draw on Thermo Fisher Scientific's bioinformatics assay design pipeline to help ensure high specificity and minimal cross-reactivity, even for gene variants with high sequence homology
  • Ideal for multiplexing—combine one FAM-labeled and one VIC-labeled assay to create a duplex assay for two different gene targets in the same qPCR well

Approximate ship time

Made to order: 4–6 days in North America, 6–10 days in Europe

Pre-formulated assay

  • 2 unlabeled PCR primers (900 nM each final 1X concentration)
  • 1 VIC dye-labeled TaqMan MGB probe (250 nM final 1X concentration)

TaqMan Gene Expression assays are the gold standard in real-time PCR gene expression studies, built on more than 20 years of experience. Each assay includes target primers and a sequence-specific probe optimized for the best functional performance. No additional design, optimization, or melt curve analysis is needed. Available in a wide variety of formats and species, new assay designs are constantly added to help meet your research needs. TaqMan assays have been cited in over 40,000 publications and are backed by more than 350 patents. All of our predesigned TaqMan Gene Expression assays are covered by the TaqMan Assays qPCR Guarantee.*

Recommended master mix:TaqMan Fast Advanced Master Mix (Cat. No. 4444556)

*Subject to terms and conditions. For complete details, go to www.thermofisher.cn/taqmanguarantee.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Fidelity (vs. Taq)60 X
For Use With (Equipment)7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System
FormatTube
Green FeaturesSustainable packaging
No. of Reactions360 reactions
Product LineTaqMan™
Product TypeGene Expression Assay
QuantityS (360 reactions), made to order
Shipping ConditionRoom Temperature
Concentration20X
Detection MethodPrimer-probe
GC-Rich PCR PerformanceHigh
PCR MethodqPCR
Reaction SpeedFast
Unit SizeEach
Contents & Storage
1 tube containing a 20X (S and M sizes) or 60X (L size) mix of pre-formulated assay (1 probe and 2 primers).

Store at -15 to -25°C.

Frequently asked questions (FAQs)

Can I multiplex my assays if my amplicons are not all the same length?

Yes, it is possible. The amplicons do not have to be the same length in order to duplex the assays. You need to use different reporter dyes on the probes in multiplex - they should have a substantial difference in maximum emission wavelength to be effective. A classic example is to use FAM and VIC for the 2 probes. Depending on the expression of the targets, you may also need to limit the primer concentration for one of the assays. This is common when duplexing a target and endogenous control (the control probe is VIC labeled and the primer concentration is decreased so the target assay can properly compete for use of enzyme and dNTPs).

Can I check the end product of a TaqMan Gene Expression Assay by running an agarose gel?

Yes, you can check the end product on an agarose gel. If you use the TaqMan Universal PCR Master Mix (including AmpErase UNG, Cat. No. 4304437), we recommend running the gel immediately after the PCR, since the trace amount of AmpErase UNG may digest the end product that contains double-stranded DNA with dUTP incorporated. The average length of amplicon ranges from 50-150 bp. The exact amplicon length is indicated on the detailed page of each assay on our website. You can get to that page by clicking on the Assay ID; the amplicon information is listed in the section "Assay Details".

Can I set up and store the TaqMan Fast Universal PCR Master Mix at room temperature if the instrument is in use?

For optimal results, we recommend to run the reaction plate right after setting up the reaction. If a reaction plate cannot be run within 2 hours of completing the reaction setup, then freeze/refrigerate the reaction plate until it can be loaded and run on the fast PCR instrument platform.

Can I run a reaction made with the standard TaqMan Universal PCR Master Mix in fast thermal cycling mode?

No, we do not recommend this approach. Since the standard TaqMan Universal PCR Master Mix is not optimized for running with fast thermal cycling, assay results will be significantly compromised.

Is the TaqMan Fast Universal PCR Master Mix offered with AmpErase UNG?

The TaqMan Fast Universal PCR Master Mix is provided without any UNG and you should add in AmpEraseUNG to reactions separately, with the addition of an initial 2 minute cycling step at 50º C. If you prefer to have a master mix with the UNG pre-added, please use TaqMan Fast Advanced Master Mix.

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