Custom TaqMan™ Gene Expression Assay, VIC
Custom TaqMan™ Gene Expression Assay, VIC
Applied Biosystems™

Custom TaqMan™ Gene Expression Assay, VIC

Applied Biosystems Custom TaqMan Gene Expression assays, VIC, are the custom version of our predesigned TaqMan assays for quantitative real-timeRead more
Have Questions?
Change viewbuttonViewtableView
Catalog NumberQuantity
4448509M (750 reactions), made to order
4448508S (360 reactions), made to order
4448510L (2900 reactions), made to order
Catalog number 4448509
Price (CNY)
5,733.00
Each
Order now
Quantity:
M (750 reactions), made to order

Applied Biosystems Custom TaqMan Gene Expression assays, VIC, are the custom version of our predesigned TaqMan assays for quantitative real-time PCR. Custom TaqMan Gene Expression assays consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (VIC) on the 5’ end and a minor groove binder (MGB) and nonfluorescent quencher (NFQ) on the 3’ end.

Features include:
• Easy to use—just add cDNA and master mix and run the qPCR—no melt curves required
• Specific—TaqMan assays use proprietary MGB-containing probes that can be up to 15 bases shorter than non-MGB probes, improving the specificity of the assay
• Sensitive—TaqMan assays are ideal for measuring low levels of expression or low-abundance targets
• Accurate—identify small fold-changes with high accuracy of quantitation
• Gold-standard TaqMan qPCR chemistry—TaqMan assays draw on Thermo Fisher Scientific's bioinformatics assay design pipeline to help ensure high specificity and minimal cross-reactivity, even for gene variants with high sequence homology
• Ideal for multiplexing—combine one FAM-labeled and one VIC-labeled assay to create a duplex assay for two different gene targets in the same qPCR well
• Sequences provided—with every Custom TaqMan Gene Expression you design and order

Approximate ship time
Made to order: 4–6 days in North America, 6–10 days in Europe

Pre-formulated assay
• 2 unlabeled PCR primers (900 nM each final 1X concentration)
• 1 VIC dye-labeled TaqMan MGB probe (250 nM final 1X concentration)

TaqMan Gene Expression assays are the gold standard in real-time PCR gene expression studies, built on more than 20 years of experience. Each assay includes target primers and a sequence-specific probe optimized for the best functional performance. No additional design, optimization, or melt curve analysis is needed. Available in a wide variety of formats and species, new assay designs are constantly added to help meet your research needs. TaqMan assays have been cited in over 40,000 publications and are backed by more than 350 patents.

Specifications
For Use With (Equipment)7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex
No. of Reactions750 reactions
PrimerLabeled PCR
Product LineTaqMan
Product TypeGene Expression Assay
QuantityM (750 reactions), made to order
Shipping ConditionRoom Temperature
Concentration20X
Unit SizeEach
Contents & Storage
1 Tube containing a 20 x (S and M sizes) or 60 x (L size) mix of pre-formulated assay (1 probe and 2 primers).

Store (at -15°C to -25°C.

)

Frequently asked questions (FAQs)

Can I check the end product of a TaqMan Gene Expression Assay by running an agarose gel?

Yes, you can check the end product on an agarose gel. If you use the TaqMan Universal PCR Master Mix (including AmpErase UNG, Cat. No. 4304437), we recommend running the gel immediately after the PCR, since the trace amount of AmpErase UNG may digest the end product that contains double-stranded DNA with dUTP incorporated. The average length of amplicon ranges from 50-150 bp. The exact amplicon length is indicated on the detailed page of each assay on our website. You can get to that page by clicking on the Assay ID; the amplicon information is listed in the section "Assay Details".

How do I select a TaqMan Gene Expression Assay for an endogenous control in relative quantitative PCR (qPCR)?

An endogenous control gene is expressed at a consistent level in all studied conditions. It is impossible to predict exactly how any gene will behave under a given range of conditions. The best way of selecting the most appropriate control gene for a relative qPCR experiment is to select some candidate genes and determine their expression levels across the range of experimental conditions and treatments. The genes most stably expressed across these conditions will be the most appropriate controls. See the following links on selection and usage. Check the TaqMan Endogenous Control Assay Selector Tool here.

Find additional tips, troubleshooting help, and resources within our Gene Expression Support Center

Can I perform a one-step RT-PCR reaction using TaqMan Gene Expression Assays?

While TaqMan Gene Expression Assays can be used to perform a one-step RT-PCR reaction, these assays have not been optimized for this application. TaqMan Gene Expression Assays have been designed, tested, and optimized using Applied Biosystems universal conditions for a two-step RT-PCR reaction.

When preparing the TaqMan or Custom TaqMan Gene Expression Assays, what is the recommended reaction volume?

When preparing the TaqMan or Custom TaqMan Gene Expression Assays, the recommended and supported volume is 20-50 µL for a 96-well standard plate set-up; 10-20 µL for a 96-well fast plate set-up and 5-10 µL for a 384-well set-up. For more information, please consult the TaqMan Gene Expression Assays and Custom TaqMan Gene Expression Assays protocols.

Find additional tips, troubleshooting help, and resources within our TaqMan Primers and Probes Support Center.

How much cDNA is recommended per TaqMan or Custom TaqMan Gene Expression reaction?

For optimal performance of the TaqMan and Custom TaqMan Gene Expression Assays, use 1 to 100 ng of cDNA per 50 µL total reaction volume. For more information, please consult the TaqMan Gene Expression Assay protocol and Custom TaqMan Gene Expression Assay protocol.

Find additional tips, troubleshooting help, and resources within our TaqMan Primers and Probes Support Center.

View all Custom TaqMan™ Gene Expression Assay, VIC, M (750 reactions), made to order FAQs