Silencer™ Select MALAT1 Positive Control siRNA

Silencer™ Select MALAT1 Positive Control siRNA is extensively validated and an ideal control for many aspects of an siRNA experiment.Read more

Catalog Number	Quantity
4455877	5 nmol

Catalog number 4455877

Price (CNY)

4,020.00

Add to cart

Quantity:

5 nmol

Price (CNY)

4,020.00

Add to cart

Silencer™ Select MALAT1 Positive Control siRNA is extensively validated and an ideal control for many aspects of an siRNA experiment. 5 nmol is provided.

• Premium-quality siRNA, highly purified and ready to use
• Functionally tested in several common cell lines
• Include Silencer™ Select modifications for enhanced specificity
• For use in human, mouse, and rat cells

What are Silencer™ Select siRNAs
Silencer™ Select siRNAs incorporate the latest improvements in siRNA design, off-target effect prediction algorithms, and chemical modifications, to yield siRNAs with unrivalled efficacy, potency, and specificity. The result is fewer failed experiments due to poor silencing, and cleaner, more consistent phenotypic data.

Silencer™ Select MALAT1 Positive Control siRNA
The Silencer™ MALAT1 positive control is extensively validated and an ideal “test” siRNA for those just beginning siRNA experiments, because it is validated to work in multiple cell lines. Because it targets MALAT1 mRNA, many assays are available to measure its effects, including real-time RT-PCR

Quality Control:
Silencer™ Select siRNAs are manufactured in a state-of-the-art facility under the highest quality standards. Rigorous quality control procedures include MALDI-TOF mass spectrometry analysis and analytical HPLC to monitor purity. Each siRNA is also assessed by gel electrophoresis to confirm that the strands anneal properly.

Accessory Product: siPORT™ NeoFX™ Transfection Agent (SKU #AM4510 and #AM4511)
siPORT™ NeoFX™ Transfection Agent is a versatile, lipid-based transfection agent for the efficient transfection of siRNA into cultured cells. The protocol can be adapted to a wide range of cells and experimental designs, including high-throughput applications.

Silencer™ Select siRNA controls have specific chemical modifications; they are designed to be used with Silencer™ Select siRNAs. If you are using other siRNA products, such as BLOCK-iT™ siRNAs, Ambion™ Silencer™ siRNAs, or STEALTH RNAi™ siRNAs, we recommend that you use control siRNAs designed for use with those siRNAs for more reliable experimental results.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Application)Transfection, RNAi

Label or DyeUnconjugated

Product LineSilencer Select, Ambion

Product TypesiRNA

PurityHPLC

Quantity5 nmol

Shipping ConditionDry Ice

Control TypePositive Control

RNAi TypesiRNA

TargetMALAT1

Unit SizeEach

Contents & Storage

The siRNA is provided dried down in a single tube, along with Nuclease-free Water for resuspension, and should be stored at –20°C.

Frequently asked questions (FAQs)

Is there a positive control for siRNA which target non-coding RNA? How about a negative control?

We do have a positive control siRNA targeting MALAT-1, which is a ncRNA localized mostly in the nucleus (Cat. No. 4455877). Silencer Select Negative Controls #1 and 2 can be used as the negative controls.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

How critical is it to include positive and negative controls in siRNA experiments?

A positive control siRNA such as the Silencer Select GAPDH siRNA will demonstrate efficient transfection and help to optimize transfection conditions. A negative control siRNA is necessary to serve as a baseline for gene expression.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

What are the positive and negative controls I should use with my Silencer or Silencer Select siRNA experiment?

We offer several negative and positive controls for both Silencer and Silencer Select siRNA.

For Silencer siRNA positive control:
- Silencer Firefly Luciferase (GL2 + GL3) siRNA (Cat. No. AM4629, 5nmol)
- Silencer GAPDH siRNA (human) (Cat. No AM4605, 5nmol; AM4633, 40 nmol)
- Silencer GAPDH siRNA (human, mouse, rat) (Cat. No. AM4624, 5 nmol; AM4631, 40 nmol; AM4632, 5 x 40 nmol)
- Silencer GFP (eGFP) siRNA (Cat. No. AM4626, 5 nmol)
- Silencer KIF11 (Eg5) siRNA (human, mouse, rat) (Cat. No. AM4639, 5 nmol)

If working with Fluorescently labeled Silencer siRNA:
- Silencer Cy-3 labeled GAPDH siRNA (human, mouse, rat) (Cat. No. AM4649, 5 nmol)
- Silencer FAM-labeled GAPDH siRNA (human, mouse, rat) (Cat. NO. AM4650, 5 nmol)

If working with Silencer siRNA in vivo ready:
- Silencer GAPDH Positive Control siRNA, in vivo ready (Cat. No. 4404025, 250 nmol)

For Silencer siRNA negative controls:
- Silencer Negative Control No. 1 siRNA (Cat. Nos. AM4611, 5 nmol; AM4635, 40 nmol; AM4636, 5 x 40 nmol)
- Silencer Negative Control No. 2 siRNA (Cat. Nos. AM4613, 5 nmol; AM4637, 30 nmol)
- Silencer Negative Control No. 3 siRNA (Cat. No. AM4615, 5 nmol)
- Silencer Negative Control No. 4 siRNA (Cat. No. AM4641, 5 nmol)
- Silencer Negative Control No. 5 siRNA (Cat. No. AM4642, 5 nmol)

If working with Fluorescently labeled Silencer siRNA:
- Silencer Cy 3-labeled Negative Control No. 1 siRNA (Cat. No AM4621, 5 nmol)
- Silencer FAM-labeled Negative Control No. 1 siRNA (Cat. No. AM4620, 5 nmol)

If working with Silencer siRNA in vivo ready:
- Silencer in vivo ready (Cat. No. 4404021)

For Silencer Select siRNA Positive control:
- Silencer Select GAPDH Positive Control siRNA (Cat. No. 4390849, 5 nmol; 4390850, 40 nmol; 4404024, 250 nmol)
- Silencer Select MALAT1 Positive Control siRNA for non-coding siRNA (Cat. No. 4455877, 5 nmol)

For Silencer Select siRNA negative control:
- Silencer Select Negative Control No. 1 siRNA (Cat. No. 4390843, 5 nmol; 4390844, 40 nmol)
- Silencer Select Negative Control No. 2 siRNA (Cat. No. 4390846, 5 nmol; 4390847, 40 nmol)

If working with Silencer Select siRNA in vivo ready:
- Silencer Select Negative Control No. 1 siRNA, in vivo ready (Cat. No. 4404020, 250nmol)

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.