Ion Plus Fragment Library Adapters

The Ion Plus Fragment Library Adapters consist of standard fragment library adapter oligos for library construction of 10-20 DNA librariesRead more

Catalog Number	Quantity
4476340	10 Reactions

Catalog number 4476340

Price (CNY)

Quantity:

10 Reactions

The Ion Plus Fragment Library Adapters consist of standard fragment library adapter oligos for library construction of 10-20 DNA libraries (depending on input DNA type and amount) for semiconductor sequencing. While library-creation kits like the Ion Plus Fragment Library Kit and Ion Xpress Plus Fragment Library Kit include standard library adapters, the need for additional adapters can sometimes arise. For example, these Ion Plus Fragment Library Adapters are suitable for use with the AB Library Builder™ System to construct automated fragment libraries. Additionally, the Long Mate-Pair Library protocol for the Ion PGM™ Sequencer requires users to purchase standard adapter oligos – a task made easy with the introduction of our Ion Plus Fragment Library Adapters.

The Ion Plus Fragment Library Adapters offer you:

• Amplification primers for PCR amplification of standard Ion Plus fragment libraries
• The convenience of premixed and appropriately diluted adapter oligos for use in Ion fragment library construction protocols including automated library construction on the AB Library Builder™ System

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Application)Sequencing

For Use With (Equipment)Ion PGM™ System

LibrariesFragment Library

No. of Reactions10

Product LineIon Torrent

Product TypeIon Plus Fragment Library Adapters

Quantity10 Reactions

Sample TypeDNA

Shipping ConditionDry Ice

Workflow StepLibrary Generation

Sequencing TypeGenome and DNA Sequencing

Unit SizeEach

Contents & Storage

4 tubes; store -20°C

Frequently asked questions (FAQs)

Are there Ion Torrent fragment library kits available for use with my AB Library Builder System?

The currently available kits for the AB Library Builder System instrument includes:

- Ion Plus Library Kit for AB Library Builder System (Cat. No. 4477597)
- Ion Xpress Plus Library Kit for AB Library Builder System (Cat. No. 4477598).

Both kits require the use of the separately available Ion Plus Fragment Library Adapters Kit (Cat. No. 4476340) or the Ion Xpress Barcode Adapters kits and the Ion Plus and Ion Xpress Plus Protocol Card for AB Library Builder System (Cat. No. 4477687).

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What is the difference between a DNA fragment library and a DNA amplicon library?

A DNA fragment library is constructed from whole genomic DNA and is commonly used for whole genome resequencing or de novo sequencing. Briefly, the whole genomic DNA is fragmented or sheared, ligated with Ion-specific adapter sequences, and then size-selected for the library fragments of the desired length.

Amplicon libraries are constructed from PCR-amplified DNA fragments and are used for targeted sequencing (e.g., investigating variants at known genomic locations). There are two types of amplicon libraries, short and long.

A short amplicon library contains DNA fragments (targets) with lengths that are compatible with the Ion template preparation kits without any further shearing or fragmentation during library preparation. Additionally, no size-selection step is required, as the amplicons are already within the desired size range.

A long amplicon library contains DNA fragments (targets) with lengths that are longer than those compatible with the Ion template kits and requires further shearing or fragmentation during library preparation. The library preparation protocol for long amplicons is similar to fragment libraries.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

The Bioanalyzer instrument trace of my fragment library shows a large peak at 70 bp or 90 bp. What can cause this? Is it okay to proceed to template preparation?

The ~70 bp or ~90 bp peak is likely standard or barcoded adapter dimers, respectively. Adapter dimers may form during the adapter ligation step and are usually removed during the size selection process. The adapter dimers will amplify on the Ion Torrent Ion Sphere particles during template preparation and decrease the overall throughput of usable sequencing reads; thus, we highly recommend removing the adapter dimers by performing an additional clean-up step prior to template preparation.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

I would like to perform Next-Generation sequencing but my final library yield is poor. What are some tips for improving my yield?

In addition to input RNA quality and accurate quantification, the clean-up and size selection steps are critical to generating a successful RNA-Seq library.

- Be sure to mix the nucleic acid binding beads well before dispensing, and follow the workflow and incubation times as closely as possible.
- Use fresh ethanol and pre-wet pipette tips prior to transferring ethanol, as the volume is critical for size selection.
- Remove residual ethanol before elution using a small-volume pipette. Do not over-dry or under-dry the beads.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

I want to prepare a DNA fragment library for Next-Generation sequencing. Which library preparation kit and protocol should I use?

We recommend using the Ion Torrent Ion Xpress Plus Fragment Library Kit (Cat. No. 4471269) for preparing libraries using the Ion Shear enzymatic shearing method, or the Ion Plus Fragment Library Kit (Cat. No. 4471252) if using physical fragmentation. The protocol for both library kits is included in the Ion Xpress Plus gDNA Fragment Library Preparation User Guide (Pub. No. 4471989). Please also refer to the Decision Tree for DNA Sequencing on the Ion PGM System and the Decision Tree for the Ion Proton System for more information.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.